Soguktas Hatice, Yaykasli Kursat Oguz, Turan Hakan, Kaya Ertugrul, Yaykasli Emine
Department of Medical Biology and Genetics, Duzce University Institute of Health Science, Duzce, Turkey.
Department of Medical Genetics, Duzce, Duzce University Medical Faculty, Turkey.
Cell Mol Biol (Noisy-le-grand). 2018 Sep 30;64(12):81-86.
Acne vulgaris is the most frequent and multifactorial inflammatory skin disorder in all races. Obesity is considered to be a risk factor for acne due to its contribution to inflammation. The involvements of inflammatory (leptin and resistin) and anti-inflammatory (adiponectin) adipokines in the pathogenesis of acne were reported. Omentin resembles adiponectin in terms of having inhibitory effect on tumor necrosis factor-α (TNF-α) induced inflammation, a vital process in the acne formation. This study was designed to investigate the putative involvement of omentin in acne formation. The genotyping was performed by restriction fragment length polymorphism (RFLP) method. Serum omentin protein levels were analyzed by enzyme-linked immunosorbent assay (ELISA). Serum omentin level was not significantly changed between groups. However, the decreased serum omentin level was observed as the mean value of BMI increased. The Asp/Asp, Val/Asp and Val/Val genotypes distributions for control and patient groups (19[17.4%], 22[20.2%], and 3[2.8%] respectively, vs. 31[28.4%], 25[22.9%], and 9[8.3%], respectively) were obtained. The Val/Val (mutant homozygote) genotype was found nearly 1.8 times more in the patient group (p=0.403, OR=1.839 (0.442-7.653)). This is the first time to clarify a linkage between anti-inflammatory omentin and acne vulgaris. Omentin Val109Asp polymorphism affects the overall function of the protein. In conclusion, omentin Val/Val (mutant homozygote) genotype increases predisposition to acne vulgaris by probably disrupting overall protein function of omentin.
寻常痤疮是所有种族中最常见且多因素导致的炎症性皮肤病。肥胖因其对炎症的影响被认为是痤疮的一个风险因素。有报道称炎症性（瘦素和抵抗素）及抗炎性（脂联素）脂肪因子参与了痤疮的发病机制。网膜素在对肿瘤坏死因子-α（TNF-α）诱导的炎症具有抑制作用方面与脂联素相似，而这种炎症是痤疮形成中的一个关键过程。本研究旨在探究网膜素在痤疮形成中的潜在作用。基因分型采用限制性片段长度多态性（RFLP）方法进行。血清网膜素蛋白水平通过酶联免疫吸附测定（ELISA）进行分析。两组之间血清网膜素水平无显著变化。然而，随着体重指数（BMI）平均值的增加，观察到血清网膜素水平下降。获得了对照组和患者组的Asp/Asp、Val/Asp和Val/Val基因型分布（分别为19[17.4%]、22[20.2%]和3[2.8%]， vs. 分别为31[28.4%]、25[22.9%]和9[8.3%]）。发现患者组中Val/Val（突变纯合子）基因型的出现频率几乎是对照组的1.8倍（p = 0.403，比值比（OR）= 1.839（0.442 - 7.653））。这是首次阐明抗炎性网膜素与寻常痤疮之间的联系。网膜素Val109Asp多态性影响该蛋白的整体功能。总之，网膜素Val/Val（突变纯合子）基因型可能通过破坏网膜素的整体蛋白功能增加患寻常痤疮的易感性。
