Kiehl A R, Fettman M J, Quackenbush S L, Hoover E A
Am J Vet Res. 1987 Jan;48(1):76-80.
A procedure for measuring in vitro feline neutrophil chemotaxis was developed, using a modified Boyden chamber apparatus and 3-microns-pore polycarbonate filters. A pooled feline serum sample was used as the chemoattractant. Chemotaxis was evaluated in 5 groups of cats: group 1-specific-pathogen-free cats that had not been exposed to feline leukemia virus (FeLV); group 2-previremic, FeLV-infected, specific-pathogen-free cats; group 3-FeLV-viremic, subclinically affected cats; group 4-FeLV-viremic, clinically affected cats; and group 5-sick cats that were not infected with FeLV. Neutrophils from the viremic, clinically affected cats had significantly lower (P less than 0.025) chemotactic responses than did those from subclinically affected, viremic cats. Conversely, neutrophils from cats that were ill due to causes other than FeLV had the highest mean chemotactic values. Among the viremic, subclinically affected cats, a linear relationship was found between age and chemotaxis, indicating that impairment of neutrophil function may be greater in younger viremic cats. However, FeLV-infected cats can not be identified on the basis of neutrophil chemotaxis.
开发了一种使用改良的博伊登室装置和3微米孔径聚碳酸酯滤膜来测量猫体外中性粒细胞趋化性的方法。使用混合猫血清样本作为趋化剂。对5组猫的趋化性进行了评估：第1组为未接触猫白血病病毒（FeLV）的无特定病原体猫；第2组为感染FeLV但处于病毒血症前期的无特定病原体猫；第3组为FeLV病毒血症且亚临床感染的猫；第4组为FeLV病毒血症且临床感染的猫；第5组为未感染FeLV的病猫。病毒血症且临床感染的猫的中性粒细胞趋化反应显著低于（P<0.025）亚临床感染且病毒血症的猫。相反，因FeLV以外原因患病的猫的中性粒细胞平均趋化值最高。在病毒血症且亚临床感染的猫中，发现年龄与趋化性之间存在线性关系，表明年轻的病毒血症猫中性粒细胞功能受损可能更严重。然而，不能根据中性粒细胞趋化性来识别FeLV感染的猫。
