Amann J F, Smith R M, Ganjam V K, Paull W K, McClure R C, Green E M, Garner H E
Am J Vet Res. 1987 Feb;48(2):323-7.
The distribution of cells that stain positive for beta-endorphin and ACTH immunoreactivity was studied in the pars intermedia (PI) of the hypophysis in 3 healthy horses and 2 healthy ponies. Serial sections treated with commercial antibodies generated against beta-endorphin or ACTH were processed for immunocytochemical studies, using the avidin biotin immunoperoxidase-complex method. Distribution patterns of cells reacting with antibodies were similar in cells from all equids. Cells immunostained for ACTH were numerous and widely distributed in the PI. Cells immunopositive for ACTH probably contain corticotrophin-like intermediate lobe peptide that cross-reacts with antisera to ACTH. Cells immunopositive for beta-endorphin were fewer in number and had a more limited distribution in the PI. Most beta-endorphin-positive cells were located along the border of the PI adjacent to the lobus nervosus and had abundant eosinophilic cytoplasm when stained with hematoxylin and eosin. Cells with prominent eosinophilic cytoplasm were not common in other areas of the PI. When serial sections were examined, cells that stained positive for beta-endorphin immunoreactivity also appeared positive for ACTH.
在3匹健康马和2匹健康矮种马的垂体中间部（PI）中，研究了β-内啡肽和促肾上腺皮质激素免疫反应性染色阳性的细胞分布。使用抗β-内啡肽或促肾上腺皮质激素的商业抗体处理连续切片，采用抗生物素蛋白-生物素免疫过氧化物酶复合物法进行免疫细胞化学研究。在所有马科动物的细胞中，与抗体反应的细胞分布模式相似。促肾上腺皮质激素免疫染色的细胞数量众多，广泛分布于垂体中间部。促肾上腺皮质激素免疫阳性的细胞可能含有与促肾上腺皮质激素抗血清发生交叉反应的促肾上腺皮质激素样中间叶肽。β-内啡肽免疫阳性的细胞数量较少，在垂体中间部的分布更为局限。大多数β-内啡肽阳性细胞位于垂体中间部与神经叶相邻的边界处，用苏木精和伊红染色时，其细胞质富含嗜酸性粒细胞。细胞质嗜酸性突出的细胞在垂体中间部的其他区域并不常见。当检查连续切片时，β-内啡肽免疫反应性染色阳性的细胞对促肾上腺皮质激素也呈阳性。
