Uva B, Vallarino M
Acta Histochem. 1985;76(1):81-7. doi: 10.1016/S0065-1281(85)80041-6.
By using immunohistochemical techniques (indirect immunofluorescence and PAP) adrenocorticotropic-like hormone (ACTH), alpha-melanocyte stimulating-like hormone (alpha-MSH), beta-lipotropic-like hormone (beta-LPH), and beta-endorphin-like (beta-EP) have been localized in the pituitary gland of newborn and adult Vipera aspis. The immunolocalization have been compared with the classical histochemical staining and the data show that the opiomelanocorticotropic-like cells resembles the B3 cells of the current literature. In the intermediate lobe both in adults and in newborns the same cells exhibit immunoreactivity for all the peptides considered, while in the rostral region of the pars distalis a difference has been observed between adults and newborns. In the adults, the cells immunostain for ACTH, beta-LPH, and beta-EP but not for alpha-MSH while in the newborns, the same cells show immunoreactivity for ACTH, beta-LPH, alpha-MSH but not for beta-EP. It has been hypothesized that in Vipera during maturation the pars distalis and the intermediate lobe can differently process the common precursor molecule.
通过使用免疫组织化学技术（间接免疫荧光和PAP法），促肾上腺皮质激素样激素（ACTH）、α-促黑素细胞激素样激素（α-MSH）、β-促脂解激素样激素（β-LPH）和β-内啡肽样激素（β-EP）已在新生和成年角蝰的垂体中定位。已将免疫定位结果与经典组织化学染色结果进行比较，数据表明阿片黑素皮质激素样细胞类似于当前文献中的B3细胞。在成年和新生动物的中间叶，相同的细胞对所有所研究的肽均表现出免疫反应性，而在远侧部的吻侧区域，成年和新生动物之间观察到差异。在成年动物中，细胞对ACTH、β-LPH和β-EP呈免疫染色阳性，但对α-MSH呈阴性，而在新生动物中，相同的细胞对ACTH、β-LPH、α-MSH呈免疫反应性，但对β-EP呈阴性。据推测，在角蝰成熟过程中，远侧部和中间叶对共同前体分子的加工方式可能不同。
