McDonnell J J, Frappier B L, Amann J F
Department of Veterinary Biomedical Sciences, University of Missouri, Columbia 65211.
Anat Rec. 1994 Dec;240(4):528-36. doi: 10.1002/ar.1092400410.
Beta-endorphin and oxytocin immunocytochemical localization were examined in the neurointermediate lobe (lobus nervosus and pars intermedia) of the bovine hypophysis in order to describe the anatomical distribution of these two neurointermediate lobe hormones.
Twenty-seven bovine hypophyses were collected from slaughterhouse animals (seven mature lactating cows, eleven mature nonlactating cows, three nulliparous heifers, and six steers). Hypophyses were immunostained for oxytocin-containing fibers and beta-endorphin-secreting cells by using the avidin biotin-immunoperoxidase method. The distributions of beta-endorphin-positive cells and oxytocin-positive nerve fibers were plotted on projected outlines of the hypophyses. Immunoreactive staining intensity was graded numerically as weak, moderate, or heavy by three individuals who had no knowledge of the animals' physiological status.
Oxytocin immunoreactivity was confined to the lobus nervosus while beta-endorphin staining was confined to the pars intermedia and the pars distalis. However, oxytocin immunopositive neurosecretory terminals were distributed more heavily in that part of the lobus nervosus bordering the pars intermedia than in the center of the lobe.
These results were similar to those previously reported for the rat (Swaab et al., 1975; J. Neural Transm., 36:195-215; Deftos and Catherwood, 1980; Life Sci., 27:223-228).
为描述牛垂体神经中间叶（神经叶和中间部）中这两种神经中间叶激素的解剖分布，对β-内啡肽和催产素进行了免疫细胞化学定位研究。
从屠宰场动物中收集了27个牛垂体（7头成熟泌乳母牛、11头成熟非泌乳母牛、3头未产小母牛和6头公牛）。采用抗生物素蛋白-生物素免疫过氧化物酶法对垂体进行含催产素纤维和分泌β-内啡肽细胞的免疫染色。将β-内啡肽阳性细胞和催产素阳性神经纤维的分布绘制在垂体的投影轮廓上。由三名不了解动物生理状态的人员将免疫反应染色强度数字分级为弱、中或强。
催产素免疫反应局限于神经叶，而β-内啡肽染色局限于中间部和远侧部。然而，催产素免疫阳性神经分泌终末在神经叶与中间部相邻的部分比在叶的中心分布更密集。
这些结果与先前报道的大鼠结果相似（斯瓦布等人，1975年；《神经传递杂志》，36:195 - 215；德夫托斯和凯瑟伍德，1980年；《生命科学》，27:223 - 228）。
