鉴定负责携带耐药因子Rts 1的宿主细胞温度敏感生长的Rts 1 DNA片段。
Identification of the Rts 1 DNA fragment responsible for temperature sensitive growth of host cells harboring a drug resistant factor Rts 1.
作者信息
Okawa N, Tanaka M, Finver S, Kaji A
出版信息
Biochem Biophys Res Commun. 1987 Feb 13;142(3):1084-8. doi: 10.1016/0006-291x(87)91526-9.
PMID:3030293
Abstract
We have placed a kanamycin resistance SalI fragment (3.65 Kb) from the drug resistance factor Rts1 into pUC19 and pBR322. These chimeric plasmids containing the kanamycin resistance fragment from Rts1 cause temperature sensitive growth in E coli. The orientation of the kanamycin resistance fragment in the vector plasmids does not influence this phenotype.
摘要
我们已将来自耐药因子Rts1的卡那霉素抗性SalI片段（3.65千碱基对）插入到pUC19和pBR322中。这些含有来自Rts1的卡那霉素抗性片段的嵌合质粒在大肠杆菌中会导致温度敏感型生长。载体质粒中卡那霉素抗性片段的方向并不影响这种表型。
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