Lobanok T E, Pesniakevich A G, Fomichev Iu K
Genetika. 1979;15(10):1739-45.
The ability of 13 Erwinia strains to accept, to inherit and to transmit the Rts1 factor by conjugation was studied. 11 strains accepted the Rts1 factor from Escherichia coli K-12 CSH-2 with the frequency of about 10(-7)--10(-3). The Rts1 factor was genetically stable in the Erwinia cells and was not eliminated by acriflavine and under the temperature of 37 and 42 degrees C. All the R+ exconjugants were characterized with more high degree of the resistance of kanamycin than E. coli cells harbouring the same R factor. Erwinia strains harbouring the Rts1 plasmid transferred it by conjugation into homologic (Erwinia) and heterologic (E. coli) bacteria. The study of kinetics of the transfer of the Rts1 factor in different mating systems showed that the transfer of this plasmid from R+ Erwinia into R- Erwinia and R- E. coli--in the liquid medium. It is concluded that Erwinia can be the host and the donor of the Rts1 factor.
研究了13株欧文氏菌通过接合作用接受、遗传和传递Rts1因子的能力。11株菌以约10(-7)--10(-3)的频率从大肠杆菌K-12 CSH-2接受Rts1因子。Rts1因子在欧文氏菌细胞中遗传稳定，在吖啶黄作用下以及在37和42摄氏度温度下不会消除。所有R+接合子对卡那霉素的抗性程度均高于携带相同R因子的大肠杆菌细胞。携带Rts1质粒的欧文氏菌菌株通过接合作用将其转移到同源（欧文氏菌）和异源（大肠杆菌）细菌中。对Rts1因子在不同交配系统中转移动力学的研究表明，该质粒在液体培养基中从R+欧文氏菌转移到R-欧文氏菌和R-大肠杆菌中。得出结论，欧文氏菌可以是Rts1因子的宿主和供体。
