Division of Production Systems Engineering, Muroran Institute of Technology, Muroran, Japan.
J Biomed Opt. 2018 Oct;24(3):1-6. doi: 10.1117/1.JBO.24.3.031012.
Blood coagulation is an important role in the hemostasis process. In the observation using microscopies, an aggregation structure of red blood cells indicates the degree of blood coagulation. Recently, it has been proposed that digital holographic microscopy (DHM) is a powerful tool for biomedical cell imaging on the basis of quantitative phase information. DHM has the advantage in that the real-time and three-dimensional (3-D) quantitative phase imaging can be realized in the wide field of view, which means that the 3-D morphological parameters of biological cells without a staining process are obtained in real time. We report the complete 3-D quantitative phase imaging of blood coagulation structures by optical projection tomography in a flow cytometry using DHM.
血液凝固在止血过程中起着重要作用。在显微镜观察中，红细胞的聚集结构表明了血液凝固的程度。最近，有人提出，数字全息显微镜（DHM）是基于定量相位信息的生物医学细胞成像的有力工具。DHM 的优点在于可以实时实现宽视场的三维（3-D）定量相位成像，这意味着可以实时获得未经染色处理的生物细胞的 3-D 形态参数。我们报告了使用 DHM 通过流式细胞术中的光学投影层析术对血液凝固结构进行的完整的 3-D 定量相位成像。
