原子力显微镜：从红细胞到免疫血液学。

Atomic force microscopy: From red blood cells to immunohaematology.

机构信息

Bioresource Processing Research Institute Australia (BioPRIA), Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.

School of Chemistry, Monash University, Clayton, VIC 3800, Australia..

出版信息

Adv Colloid Interface Sci. 2017 Nov;249:149-162. doi: 10.1016/j.cis.2017.05.011. Epub 2017 May 11.

DOI:10.1016/j.cis.2017.05.011

PMID:28515013

Abstract

Atomic force microscopy (AFM) offers complementary imaging modes that can provide morphological and structural details of red blood cells (RBCs), and characterize interactions between specific biomolecules and RBC surface antigen. This review describes the applications of AFM in determining RBC health by the observation of cell morphology, elasticity and surface roughness. Measurement of interaction forces between plasma proteins and antibodies against RBC surface antigen using the AFM also brought new information to the immunohaematology field. With constant improvisation of the AFM in resolution and imaging time, the reaction of RBC to changes in the physico-chemistry of its environment and the presence of RBC surface antigen specific-biomolecules is achievable.

摘要

原子力显微镜（AFM）提供了互补的成像模式，可以提供红细胞（RBC）的形态和结构细节，并表征特定生物分子与 RBC 表面抗原之间的相互作用。本综述描述了通过观察细胞形态、弹性和表面粗糙度来确定 RBC 健康的 AFM 的应用。使用 AFM 测量血浆蛋白与针对 RBC 表面抗原的抗体之间的相互作用力，也为免疫血液学领域带来了新的信息。随着 AFM 在分辨率和成像时间方面的不断改进，可以实现 RBC 对其环境物理化学变化和 RBC 表面抗原特异性生物分子存在的反应。