Nakagawa H, Kitagawa H, Aikawa Y
Biochem Biophys Res Commun. 1987 Feb 13;142(3):791-7. doi: 10.1016/0006-291x(87)91483-5.
Tumor necrosis factor (TNF, 2.6 X 10(-11)-10(-9) M) caused an increase in the production of active gelatinase and latent collagenase by granulation tissue in culture. As determined by SDS-substrate polyacrylamide gel electrophoresis, granulation tissue produced mainly two species of gelatinase with molecular weights of 64 kDa and 57 kDa, and an additional 80-kDa gelatinase was produced by TNF treatment. The results suggest that TNF may play a role in a rapid collagen turnover in the carrageenin-induced granulation tissue in rats.
肿瘤坏死因子（TNF，2.6×10⁻¹¹ - 10⁻⁹ M）可使培养中的肉芽组织产生的活性明胶酶和潜伏胶原酶增加。通过十二烷基硫酸钠 - 底物聚丙烯酰胺凝胶电泳测定，肉芽组织主要产生两种分子量分别为64 kDa和57 kDa的明胶酶，经TNF处理后还产生了一种额外的80 kDa明胶酶。结果表明，TNF可能在角叉菜胶诱导的大鼠肉芽组织中快速胶原周转中发挥作用。
