Okada Y, Tsuchiya H, Shimizu H, Tomita K, Nakanishi I, Sato H, Seiki M, Yamashita K, Hayakawa T
Department of Pathology, Kanazawa University, Japan.
Biochem Biophys Res Commun. 1990 Sep 14;171(2):610-7. doi: 10.1016/0006-291x(90)91190-4.
Production of a 92-kDa gelatinase/type IV collagenase and tissue inhibitor of metalloproteinases (TIMP) was investigated with human sarcoma cell lines. Among the cytokines and growth factors examined, only human recombinant tumor necrosis factor alpha (TNF alpha) induced and stimulated the proteinase with concomitant increase in TIMP expression, but matrix metalloproteinase 2 (72-kDa gelatinase/type IV collagenase) expression was unchanged. These data suggest that gene expression of the two metalloproteinases is regulated in a different fashion and TNF alpha may be important to allow cancer cells to be more invasive and metastatic.
利用人肉瘤细胞系研究了92-kDa明胶酶/IV型胶原酶和金属蛋白酶组织抑制剂（TIMP）的产生。在所检测的细胞因子和生长因子中，只有人重组肿瘤坏死因子α（TNFα）诱导并刺激了该蛋白酶，同时TIMP表达增加，但基质金属蛋白酶2（72-kDa明胶酶/IV型胶原酶）的表达未发生变化。这些数据表明，这两种金属蛋白酶的基因表达是以不同方式调控的，并且TNFα对于癌细胞更具侵袭性和转移性可能很重要。
