Institute of Environmental Sciences, Jagiellonian University, Kraków, Poland.
Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland.
J Basic Microbiol. 2019 Jan;59(1):24-37. doi: 10.1002/jobm.201800406. Epub 2018 Oct 10.
The aim of this study was to assess the biodiversity of endophytic fungi from Arabidopsis arenosa growing on a post mining waste dump and to evaluate their role in plant adaptation to metal toxicity. Severeal of the fungi were beneficial for the plant. Among them, a fungus belonging to the Mucor genus, was found to interact with a broad range of plants, including Brassicaceae metallophytes. Mucor sp. was shown to be highly tolerant to elevated levels of Zn, Cd, and Pb and to accelerate plant-host growth under either toxic-metal stress or control conditions. When inoculated with Mucor sp., A. arenosa under toxic-metal stress acquired more N and showed significantly down-regulated catalase activity, which suggests suppression of toxic-metal-induced oxidative stress. We used the model plant-A. thaliana to evaluate the dynamics of plant-tissue colonization by the fungus as monitored with qPCR and to analyze the host's transcriptome response during early stages of the interaction. The results revealed the induction of a plant-defense and stress-related response on the 5 day of co-culture, which was in accord with the decrease of fungal abundance in shoots on the 6 day of interaction. Presented results demonstrate the importance of endophytic fungi in plant toxic-metal tolerance.
本研究旨在评估生长在矿山废石堆上的拟南芥内生真菌的生物多样性，并评估其在植物适应金属毒性方面的作用。一些真菌对植物有益。其中，一种属于毛霉属的真菌被发现与包括十字花科金属植物在内的广泛植物相互作用。毛霉属真菌对 Zn、Cd 和 Pb 的高水平具有高度耐受性，并能在有毒金属胁迫或对照条件下加速植物宿主的生长。当接种毛霉属真菌时，在有毒金属胁迫下的拟南芥获得了更多的氮，并表现出明显下调的过氧化氢酶活性，这表明抑制了有毒金属诱导的氧化应激。我们使用模式植物拟南芥来评估真菌对植物组织的定殖动力学，并用 qPCR 进行监测，并分析在相互作用的早期阶段宿主的转录组反应。结果表明，在共培养的第 5 天诱导了植物防御和应激相关反应，这与第 6 天相互作用时茎中真菌丰度的下降相一致。目前的结果表明，内生真菌在植物耐受有毒金属方面具有重要意义。
