Department of Aquaculture, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China.
Department of Aquaculture, Putian Municipal Institute of Fisheries Research, Putian, China.
Mol Reprod Dev. 2019 Feb;86(2):132-144. doi: 10.1002/mrd.23065. Epub 2018 Nov 26.
In the present study, the next-generation sequencing technology was used to develop a transcriptome database of gonad and liver from 3-year-old male and female Amur sturgeons (Acipenser schrenckii). A total of 139,406 unigenes were generated after the Illumina Hiseq. 2500 sequence and assembled by Trinity. The differential expression analysis between male and female obtained 5,199 differentially expressed genes (DEGs) in gonad and 457 DEGs in liver. Gene Ontology enrich analysis showed that the specific DEGs of gonad play a dominant role in reproductive processes. Although the specific DEGs of liver indicated their primary responsibility for energy metabolism, the DEGs of liver and gonad co-own enriched in terms associated with reproduction suggested that liver also plays a role in sex-related differences in Amur sturgeon. Furthermore, genes related to sex-related differences were selected to validate among the four different tissues by real-time quantitative polymerase chain reaction (qRT-PCR). In addition, by trans-acting analysis, a total of 5,206 putative long noncoding RNAs (lncRNAs) and 3,490 target genes of lncRNAs were predicted from gonad and liver. Moreover, several lncRNAs targeting Mea1, Piwil1, Tdrd1, Nanos2, Ankrd49, and ZP3 may have potential regulatory effect related to gametogenesis and gonadal differentiation were identified and validated by qRT-PCR. These results suggested for the first time that lncRNAs might be one of the effect factors in regulating the differential expression of messenger RNAs associated with sex-related differences in Amur sturgeon.
在本研究中，我们使用下一代测序技术构建了 3 龄雌雄中华鲟性腺和肝脏的转录组数据库。通过 Illumina Hiseq. 2500 测序和 Trinity 组装，共获得了 139406 条 unigenes。对雌雄性腺差异表达分析获得了 5199 个差异表达基因（DEGs）和 457 个差异表达基因（DEGs）在肝脏中。基因本体论（GO）富集分析表明，性腺特异性 DEGs 在生殖过程中发挥主导作用。尽管肝脏特异性 DEGs 表明其主要负责能量代谢，但肝脏和性腺的 DEGs 共同富集与生殖相关的术语表明，肝脏在中华鲟性别差异中也发挥作用。此外，通过实时定量聚合酶链反应（qRT-PCR）在四个不同组织中选择验证与性别差异相关的基因。此外，通过反式作用分析，从性腺和肝脏共预测到 5206 个潜在的长非编码 RNA（lncRNA）和 3490 个 lncRNA 的靶基因。此外，通过 qRT-PCR 鉴定和验证了几个靶向 Mea1、Piwil1、Tdrd1、Nanos2、Ankrd49 和 ZP3 的 lncRNA 可能具有与配子发生和性腺分化相关的潜在调节作用。这些结果首次表明，lncRNA 可能是调节与中华鲟性别差异相关的信使 RNA 差异表达的影响因素之一。
