Scotto Gaetano, Fazio Vincenzina
Microbiology and Clinical Microbiology, Master's Degree in Dentistry, Faculty of Medicine and Surgery, University of Foggia, Foggia, Italy.
Public Health and Preventive Medicine, and Clinical Laboratory specialist, Foggia, Italy.
Rev Soc Bras Med Trop. 2018 Sep-Oct;51(5):578-583. doi: 10.1590/0037-8682-0430-2017.
Here, we conducted an epidemiological study of hepatitis B virus (HBV) mono-infected and asymptomatic malaria/HBV coinfected immigrants and further discussed the possibility of malaria disease modifying the clinical presentation of HBV infection.
A total of 195 African immigrants were examined for HBV infection or coinfection with HBV and asymptomatic malaria. HBV infection was diagnosed using serological tests and confirmed by PCR; furthermore, we performed a pan-Plasmodium-specific-nucleic-acid-sequence-based-amplification (NASBA) assay to detect asymptomatic malaria infection. The stage/grade of the liver disease was determined using echotomography and elastometry.
PCR-NASBA results confirmed that 62 of 195 subjects (31.8%) were positive for Plasmodium infection, whereas 41 of 195 subjects (21%) tested positive for HBV chronic hepatitis (HBV-DNA positive). Among the HBV-positive subjects, 26 (63.4%) of them were mono-infected patients (Group A), whereas 15 (36.6%) patients had HBV chronic hepatitis and asymptomatic malaria coinfections (Group B). The HBV-DNA median levels were 1.4×105IU/mL in HBV-mono-infected patients and 2.0×105IU/mL in coinfected patients. Echotomography and hepatic elastometry presented similar findings for both groups of patients.
Coinfected patients seem to present with the same clinical symptoms of the liver disease as HBV mono-infected patients.
在此，我们对乙型肝炎病毒（HBV）单感染以及无症状疟疾/HBV合并感染的移民进行了一项流行病学研究，并进一步探讨了疟疾改变HBV感染临床表现的可能性。
对总共195名非洲移民进行了HBV感染或HBV与无症状疟疾合并感染的检测。使用血清学检测诊断HBV感染，并通过PCR进行确认；此外，我们进行了基于全疟原虫特异性核酸序列扩增（NASBA）检测以检测无症状疟疾感染。使用超声断层扫描和弹性测定法确定肝脏疾病的阶段/分级。
PCR-NASBA结果证实，195名受试者中有62名（31.8%）疟原虫感染呈阳性，而195名受试者中有41名（21%）HBV慢性肝炎检测呈阳性（HBV-DNA阳性）。在HBV阳性受试者中，其中26名（63.4%）为单感染患者（A组），而15名（36.6%）患者为HBV慢性肝炎和无症状疟疾合并感染（B组）。HBV单感染患者的HBV-DNA中位水平为1.4×105IU/mL，合并感染患者为2.0×105IU/mL。两组患者的超声断层扫描和肝脏弹性测定结果相似。
合并感染患者似乎与HBV单感染患者表现出相同的肝脏疾病临床症状。
