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γδ 解离酶在重组交叉点诱导出一种异常的 DNA 结构。

The gamma delta resolvase induces an unusual DNA structure at the recombinational crossover point.

作者信息

Hatfull G F, Noble S M, Grindley N D

出版信息

Cell. 1987 Apr 10;49(1):103-10. doi: 10.1016/0092-8674(87)90760-4.

DOI:10.1016/0092-8674(87)90760-4
PMID:3030563
Abstract

gamma delta resolvase, a transposon-encoded protein active in site-specific recombination, induces a structural change in the DNA at the recombinational crossover point that results in enhanced intercalation of the foot-printing reagent MPE X Fe(II). The structural change correlates with the formation of a bend in the DNA: a mutant resolvase that binds to the crossover site but induces little or no bend does not promote intercalation. The properties of the mutant protein suggest that the induced structural change, which we propose is a localized kink, is required for recombination.

摘要

γδ 解离酶是一种在位点特异性重组中具有活性的转座子编码蛋白，它会在重组交叉点处诱导 DNA 发生结构变化，从而导致足迹试剂 MPE X Fe(II) 的嵌入增强。这种结构变化与 DNA 弯曲的形成相关：一种与交叉位点结合但几乎不诱导或不诱导弯曲的突变解离酶不会促进嵌入。突变蛋白的特性表明，我们认为这种诱导的结构变化是一种局部扭结，是重组所必需的。

相似文献

1
The gamma delta resolvase induces an unusual DNA structure at the recombinational crossover point.γδ 解离酶在重组交叉点诱导出一种异常的 DNA 结构。
Cell. 1987 Apr 10;49(1):103-10. doi: 10.1016/0092-8674(87)90760-4.
2
Transposon-mediated site-specific recombination: identification of three binding sites for resolvase at the res sites of gamma delta and Tn3.转座子介导的位点特异性重组：在γδ和Tn3的res位点鉴定出解离酶的三个结合位点。
Cell. 1982 Aug;30(1):19-27. doi: 10.1016/0092-8674(82)90007-1.
3
Uncoupling of the recombination and topoisomerase activities of the gamma delta resolvase by a mutation at the crossover point.通过交叉点处的突变使γδ解离酶的重组活性和拓扑异构酶活性解偶联。
Nature. 1988 Apr 28;332(6167):861-3. doi: 10.1038/332861a0.
4
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res.γδ 解离酶突变体的体外分析：解离酶丝氨酸-10 与 res 位点 I 之间相互作用的证据
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5429-33. doi: 10.1073/pnas.83.15.5429.
5
Site-specific recombination at res sites containing DNA-binding sequences for both Tn21 and Tn3 resolvases.在含有Tn21和Tn3解离酶的DNA结合序列的res位点处进行位点特异性重组。
J Mol Biol. 1995 Jan 20;245(3):208-18. doi: 10.1006/jmbi.1994.0017.
6
Cooperativity mutants of the gamma delta resolvase identify an essential interdimer interaction.γδ 解离酶的协同性突变体确定了一种必需的二聚体间相互作用。
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7
Catalytic residues of gamma delta resolvase act in cis.γδ 解离酶的催化残基以顺式作用。
EMBO J. 1995 Oct 16;14(20):5129-40. doi: 10.1002/j.1460-2075.1995.tb00195.x.
8
Unraveling transposition: gamma delta resolvase in complex with DNA.
Structure. 1995 Aug 15;3(8):755-8. doi: 10.1016/s0969-2126(01)00210-6.
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The two functional domains of gamma delta resolvase act on the same recombination site: implications for the mechanism of strand exchange.γδ 解离酶的两个功能结构域作用于同一个重组位点：对链交换机制的启示。
Proc Natl Acad Sci U S A. 1990 Jul;87(14):5336-40. doi: 10.1073/pnas.87.14.5336.
10
Site-specific recombination at res sites containing DNA-binding sequences for both Tn21 resolvase and CAP.在含有Tn21解离酶和CAP的DNA结合序列的res位点处进行位点特异性重组。
J Mol Biol. 1995 Jan 20;245(3):219-27. doi: 10.1006/jmbi.1994.0018.

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EMBO J. 1993 Jun;12(6):2495-501. doi: 10.1002/j.1460-2075.1993.tb05904.x.
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