APC Microbiome Ireland and School of Microbiology, University College Cork, Western Road, Cork, Ireland.
Danone Nutricia Research, Avenue de la Vauve, 91767, Palaiseau, France.
Appl Microbiol Biotechnol. 2018 Dec;102(24):10645-10663. doi: 10.1007/s00253-018-9413-7. Epub 2018 Oct 10.
Members of the Bifidobacterium genus are widely used as probiotics in fermented milk products. Bifidobacterium animalis subsp. animalis CNCM I-4602 grows and survives poorly in reconstituted skimmed milk (RSM). Availing of genome and transcriptome information, this poor growth and survival phenotype in milk was substantially improved by the addition of certain compounds, such as yeast extract, uric acid, glutathione, cysteine, ferrous sulfate, and a combination of magnesium sulfate and manganese sulfate. Carbohydrate utilization of CNCM I-4602 was also investigated, allowing the identification of several carbohydrate utilization gene clusters, and highlighting this strain's inability to utilize lactose, unlike the type strain of this subspecies, B. animalis subsp. animalis ATCC25527 and the B. animalis subsp. lactis subspecies. In addition, the ability of B. animalis subsp. animalis CNCM I-4602 to colonize a murine model was investigated, which showed that this strain persists in the murine gut for a period of at least 4 weeks. Associated in vivo transcriptome analysis revealed that, among other genes, a gene cluster encoding a predicted type IVb tight adherence (Tad) pilus was upregulated, indicating that this extracellular structure plays a role in the colonization/adaptation of the murine gastrointestinal tract by this strain.
双歧杆菌属成员被广泛用作发酵乳制品中的益生菌。动物双歧杆菌亚种。动物亚种 CNCM I-4602 在再水合脱脂乳（RSM）中生长和存活不良。利用基因组和转录组信息，通过添加某些化合物，如酵母提取物、尿酸、谷胱甘肽、半胱氨酸、硫酸亚铁和硫酸镁和硫酸锰的组合，可以显著改善这种在牛奶中的不良生长和存活表型。还研究了 CNCM I-4602 的碳水化合物利用情况，鉴定出了几个碳水化合物利用基因簇，并突出了该菌株不能利用乳糖的能力，这与该亚种的模式株不同，动物双歧杆菌亚种。动物亚种 ATCC25527 和动物双歧杆菌亚种。此外，还研究了动物双歧杆菌亚种。动物亚种 CNCM I-4602 在小鼠模型中的定植能力，结果表明该菌株至少在 4 周内持续存在于小鼠肠道中。相关的体内转录组分析表明，除其他基因外，一个编码预测的 IVb 型紧密附着（Tad）菌毛的基因簇被上调，表明这种细胞外结构在该菌株对小鼠胃肠道的定植/适应中发挥作用。
