Lausch R N, Lee J D, Oakes J E
Curr Eye Res. 1987 Jan;6(1):27-32. doi: 10.3109/02713688709020064.
An intertypic recombinant constructed from HSV-1 x HSV-2 parents was isolated which failed to induce any overt ocular pathology when inoculated onto the sacrificed cornea of four-week-old SJL/J mice. During the 24-48 hour post-infection period there was transient virus replication but by day 3 the infectious titer in the eye had dropped by greater than or equal to 10(4)-fold, and little or no virus could be recovered thereafter. When immunosuppressed (600 r) mice were infected corneally, virus clearance was delayed several days but again no obvious ocular pathology was seen, and no mice died. By contrast, infection of the cornea with either parent was followed by virus replication and development of clinically apparent pathology which could progress to blinding stromal keratitis. The genome of the intertypic recombinant was analyzed by agarose gel electrophoresis of restriction endonuclease digests and found to consist entirely of HSV-1 DNA except for HSV-2 DNA sequences located between map units 0.10-0.16, 0.41-0.43, and 0.77-1.0. Potential explanations for the loss of virulence are discussed.
从单纯疱疹病毒1型（HSV - 1）和单纯疱疹病毒2型（HSV - 2）亲本构建的一种型间重组体被分离出来，当将其接种到四周龄SJL/J小鼠的处死角膜上时，未引发任何明显的眼部病理变化。在感染后的24至48小时内有短暂的病毒复制，但到第3天时，眼中的感染滴度下降了大于或等于10⁴倍，此后几乎无法回收或无法回收病毒。当免疫抑制（600伦琴）小鼠角膜感染时，病毒清除延迟了几天，但同样未观察到明显的眼部病理变化，且没有小鼠死亡。相比之下，用任一亲本感染角膜后都会出现病毒复制和明显的临床病理变化，这些变化可能进展为致盲性基质性角膜炎。通过对限制性内切酶消化产物进行琼脂糖凝胶电泳分析型间重组体的基因组，发现除了位于图谱单位0.10 - 0.16、0.41 - 0.43和0.77 - 1.0之间的HSV - 2 DNA序列外，其完全由HSV - 1 DNA组成。文中讨论了毒力丧失的可能原因。
