Oakes J E, Gray W L, Lausch R N
Virology. 1986 Apr 30;150(2):513-7. doi: 10.1016/0042-6822(86)90316-8.
The virulence of a herpes simplex virus (HSV) intertypic recombinant possessing HSV-1 DNA sequences from map units 0.31 to 0.44 and HSV-2 sequences from map units 0 to 0.30 and 0.45 to 1.0 were compared with the virulence of the two parental strains. Following ocular inoculation, both the intertypic recombinant and the HSV-1 parent replicated at the infection site and spread to the peripheral and central nervous system (CNS) to produce fatal encephalitis. The HSV-2 parent also replicated at the infection site but failed to progress to the CNS. However, when inoculated intracerebrally, the HSV-2 strain was as lethal as the HSV-1 parent. Furthermore, the HSV-2 strain could produce thymidine kinase at 37 and 39 degrees in levels comparable to the HSV-1 strain. The results indicate that transfer of the HSV-1 DNA sequences imparted to the recombinant virus the necessary genetic information to spread from the cornea into the central nervous system.
将具有来自图谱单位0.31至0.44的单纯疱疹病毒1型（HSV-1）DNA序列以及来自图谱单位0至0.30和0.45至1.0的HSV-2序列的HSV种间重组体的毒力与两种亲本菌株的毒力进行了比较。眼部接种后，种间重组体和HSV-1亲本均在感染部位复制，并扩散至外周和中枢神经系统（CNS），引发致命性脑炎。HSV-2亲本也在感染部位复制，但未能扩散至中枢神经系统。然而，脑内接种时，HSV-2菌株与HSV-1亲本一样具有致死性。此外，HSV-2菌株在37和39摄氏度时产生胸苷激酶的水平与HSV-1菌株相当。结果表明，HSV-1 DNA序列的转移赋予了重组病毒从角膜扩散至中枢神经系统所需的遗传信息。
