a School of Chemical Engineering , Sichuan University , Chengdu , China.
b State Key Laboratory of Oral Diseases West China College of Stomatology , Sichuan University , Chengdu , China.
J Biomater Sci Polym Ed. 2018 Dec;29(18):2237-2251. doi: 10.1080/09205063.2018.1534668. Epub 2018 Dec 7.
A strategy developed for obtaining positive cellular responses remains to be focused in the filed of functional biomimetics. In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy. Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently. In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control. Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds. Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.
一种用于获得阳性细胞反应的策略仍然专注于功能仿生学领域。在这项研究中，构建了一种载有辛伐他汀的聚醚醚酮（PEEK）生物复合材料水凝胶，用于骨组织再生治疗。简而言之，在 PEEK 表面制造了三维（3D）多孔结构；然后，用聚（L-乳酸）/辛伐他汀多孔膜和透明质酸水凝胶对基底进行功能化。体外细胞黏附、增殖和细胞骨架观察实验表明，由于透明质酸水凝胶层的存在，我们的支架表现出更好的生物亲和力，与对照组相比。此外，碱性磷酸酶活性、钙矿沉积评价和成骨潜能的基因表达均表明，MC3T3-E1 前成骨细胞在我们支架上的成骨分化能力更强。因此，我们载有辛伐他汀并覆盖透明质酸水凝胶的 PEEK 样品在骨修复的临床应用中具有很大的潜力。
