Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; Department of Orthopaedics, The Second Hospital, Jilin University, Changchun 130041, PR China; Department of Orthopaedics, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, PR China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Colloids Surf B Biointerfaces. 2018 Sep 1;169:233-241. doi: 10.1016/j.colsurfb.2018.05.027. Epub 2018 May 15.
Polyetheretherketone (PEEK) is a high-performance semicrystalline thermoplastic polymer that is widely used in the orthopedics treatment. However, due to its biological inertness, the surface modification of PEEK using different methods to improve the biocompatibility remains a significant challenge. Herein, we attempted to use the covalently coating of phosphorylated gelatin loaded with bone morphogenetic protein 2 (BMP-2) on hydroxylated micro-porous PEEK films for enhancing the biological activity. Environmental scanning electron microscope (ESEM), fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and water contact angle measurements were applied to characterize the surface of modified or untreated PEEK films. The influence on cell adhesion, proliferation and differentiation was evaluated by culturing of mouse pre-osteoblasts (MC3T3-E1) on different modified PEEK substrates in vitro. Surface characterization showed that the modification was successfully performed on PEEK films. The biological results indicated that surface modification of micro-porous PEEK using phosphorylated gelatin significantly promoted cell adhesion and proliferation. And the osteogenic differentiation was effectively improved while loading with different amounts of BMP-2. Findings from this study indicated that this novel biological modification on PEEK films might be helpful for altering its biological inertness and further expand its medical applications as a kind of orthopedic implants.
聚醚醚酮（PEEK）是一种高性能半结晶热塑性聚合物，广泛应用于骨科治疗。然而，由于其生物惰性，使用不同方法对 PEEK 进行表面改性以提高生物相容性仍然是一个重大挑战。在此，我们尝试使用载有骨形态发生蛋白 2（BMP-2）的磷酸化明胶共价涂层对羟基化微孔 PEEK 薄膜进行改性，以提高其生物活性。环境扫描电子显微镜（ESEM）、傅里叶变换红外光谱（FT-IR）、X 射线光电子能谱（XPS）和水接触角测量用于表征改性或未处理的 PEEK 薄膜的表面。通过体外培养小鼠前成骨细胞（MC3T3-E1）在不同改性 PEEK 基底上，评估对细胞黏附、增殖和分化的影响。表面特性表明成功地对 PEEK 薄膜进行了改性。生物学结果表明，使用磷酸化明胶对微孔 PEEK 的表面改性显著促进了细胞黏附与增殖。同时，负载不同量的 BMP-2 可有效促进成骨分化。本研究结果表明，这种新型 PEEK 薄膜的生物改性可能有助于改变其生物惰性，并进一步扩展其作为一种骨科植入物的医学应用。
