Abrams J, Jacobovitz D, Dumont P, Semal P, Mommen P, Klastersky J, Atassi G
Eur J Cancer Clin Oncol. 1986 Nov;22(11):1387-94. doi: 10.1016/0277-5379(86)90150-1.
The 6 day subrenal capsule (SRC) assay was performed in normal mice using 20 fresh human non-small cell lung cancers and nine fresh ovarian cancers. Different multi-agent chemotherapy regimens administered intravenously on days 2 + 3 of the assay were evaluated for activity against these two tumor types. Macroscopic results measured via an ocular microscope showed high activity for some combinations as well as for single agents. However, when subjected to microscopic examination, the SRC implants in the untreated control animals did not show viable tumor growth on day 6. Detailed histologic evaluation of over 800 SRC grafts reveals an intense inflammatory and fibrotic reaction in the majority of the grafts. These results indicate that drug sensitivity patterns obtained with this assay using only macroscopic criteria do not correlate with actual tumor regression. Microscopic analysis of the grafts prior to transplant shows absence or only minimal presence of tumor in many cases which also contributes to the poor growth observed in these two tumors.
在正常小鼠中进行了为期6天的肾包膜下（SRC）试验，使用了20例新鲜的人类非小细胞肺癌和9例新鲜的卵巢癌。评估了在试验第2天和第3天静脉给予的不同多药化疗方案对这两种肿瘤类型的活性。通过肉眼显微镜测量的宏观结果显示，某些联合用药以及单药具有高活性。然而，在显微镜检查时，未治疗的对照动物中的SRC植入物在第6天未显示存活肿瘤生长。对800多个SRC移植物进行的详细组织学评估显示，大多数移植物中存在强烈的炎症和纤维化反应。这些结果表明，仅使用宏观标准通过该试验获得的药物敏感性模式与实际肿瘤消退不相关。移植前对移植物的显微镜分析显示，在许多情况下肿瘤不存在或仅微量存在，这也导致了在这两种肿瘤中观察到的生长不良。
