一种来自噬菌体 80α 的新型释放蛋白，促进裂解生长。
A novel ejection protein from bacteriophage 80α that promotes lytic growth.
机构信息
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
出版信息
Virology. 2018 Dec;525:237-247. doi: 10.1016/j.virol.2018.09.025. Epub 2018 Oct 8.
Many staphylococcal bacteriophages encode a minor capsid protein between the genes for the portal and scaffolding proteins. In Staphylococcus aureus bacteriophage 80α, this protein, called gp44, is essential for the production of viable phage, but dispensable for the phage-mediated mobilization of S. aureus pathogenicity islands. We show here that gp44 is not required for capsid assembly, DNA packaging or ejection of the DNA, nor for generalized transduction of plasmids. An 80α Δ44 mutant could be complemented in trans by gp44 expressed from a plasmid, indicating that gp44 plays a post-injection role in the host. Our results show that gp44 is an ejection (pilot) protein that is involved in deciding the fate of the phage DNA after injection. Our data are consistent with a model in which gp44 acts as a regulatory protein that promotes progression to the lytic cycle.
许多葡萄球菌噬菌体在门户和支架蛋白基因之间编码一个次要衣壳蛋白。在金黄色葡萄球菌噬菌体 80α 中，这种称为 gp44 的蛋白质对于产生有活力的噬菌体是必需的，但对于噬菌体介导的金黄色葡萄球菌致病性岛的移动是可有可无的。我们在这里表明，gp44 不参与衣壳组装、DNA 包装或 DNA 排出，也不参与质粒的普遍转导。一个 80α Δ44 突变体可以通过从质粒表达的 gp44 进行反式互补，表明 gp44 在宿主中发挥注射后的作用。我们的结果表明，gp44 是一种排出（引导）蛋白，它参与决定注射后噬菌体 DNA 的命运。我们的数据与一种模型一致，即 gp44 作为一种调节蛋白，促进向裂解周期的进展。
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