van Leen R W, van Roozendaal K E, Lubsen N H, Schoenmakers J G
Dev Biol. 1987 Apr;120(2):457-64. doi: 10.1016/0012-1606(87)90249-1.
The concentrations of alpha A-, beta B1-, and total gamma-crystallin mRNAs were measured during development of the rat eye lens, using Northern blot and dot blot analysis. After 14 days of fetal growth a sharp increase in the concentration of all three mRNA species was observed. After birth, the concentration of alpha A-crystallin transcripts remains high until 6 months of age, the concentration of gamma-crystallin transcripts decreases gradually, while the concentration of beta B1-crystallin transcripts decreases sharply. The composition of the gamma-crystallin mRNA pool was determined by measuring the relative amount of the transcripts of each of the six gamma-crystallin genes using primer extension and S1-nuclease mapping. The transcripts of all six genes are found until the third month after birth. Thereafter the transcripts of the gamma 1-1, the gamma 3-1, and gamma 4-1 crystallin genes are no longer detectable. Later on the transcripts of the gamma 2-1 and gamma 2-2 genes also disappear leaving only the transcripts of the gamma 1-2 crystallin gene at the age of 8 months. The concentration of the six different gamma-crystallin mRNAs is thus regulated differentially.
采用Northern印迹法和斑点印迹分析法，测定大鼠晶状体发育过程中αA-、βB1-和总γ-晶状体蛋白mRNA的浓度。胎儿生长14天后，观察到所有三种mRNA的浓度急剧增加。出生后，αA-晶状体蛋白转录物的浓度在6个月龄前一直保持较高水平，γ-晶状体蛋白转录物的浓度逐渐降低，而βB1-晶状体蛋白转录物的浓度急剧下降。通过使用引物延伸和S1核酸酶作图法测定六个γ-晶状体蛋白基因各自转录物的相对量，确定γ-晶状体蛋白mRNA库的组成。在出生后第三个月之前均可发现所有六个基因的转录物。此后，γ1-1、γ3-1和γ4-1晶状体蛋白基因的转录物不再能被检测到。后来，γ2-1和γ2-2基因的转录物也消失了，到8个月龄时仅留下γ1-2晶状体蛋白基因的转录物。因此，六种不同的γ-晶状体蛋白mRNA的浓度受到不同的调节。
