Parker D S, Wawrousek E F, Piatigorsky J
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892.
Dev Biol. 1988 Apr;126(2):375-81. doi: 10.1016/0012-1606(88)90147-9.
The amounts of lens mRNA derived from the delta 1- and delta 2-crystallin genes of the chicken were determined by primer extension experiments using gene-specific synthetic oligonucleotides. The primer extended products were sequenced to establish the identity of the resulting cDNAs. The results indicated that most of the delta-crystallin mRNA in the 14-day-old embryonic lens contained transcripts derived from the delta 1 gene. Importantly, however, about 1-2% of the extended products were derived from delta 2 mRNA. Although not quantitative, the primer extension experiments suggested that the delta 1/delta 2 mRNA ratio may differ in the lens fiber cells during development between 6 days of embryogenesis and 3 weeks after hatching. These data provide the first demonstration for the presence of delta 2-crystallin mRNA in the chicken lens and raise the possibility that the two linked, extremely similar delta-crystallin genes are differentially regulated during development.
通过使用基因特异性合成寡核苷酸进行引物延伸实验，测定了源自鸡δ1-和δ2-晶状体蛋白基因的晶状体mRNA的量。对引物延伸产物进行测序以确定所得cDNA的身份。结果表明，14日龄胚胎晶状体中大部分δ-晶状体蛋白mRNA包含源自δ1基因的转录本。然而，重要的是，约1-2%的延伸产物源自δ2 mRNA。虽然不是定量的，但引物延伸实验表明，在胚胎发育6天至孵化后3周的过程中，晶状体纤维细胞中δ1/δ2 mRNA的比例可能有所不同。这些数据首次证明了鸡晶状体中存在δ2-晶状体蛋白mRNA，并增加了这两个连锁的、极其相似的δ-晶状体蛋白基因在发育过程中受到差异调节的可能性。
