Anan Hoda H, Zidan Rania A, Abd El-Baset Samia A, Ali Manar M
Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt.
Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt.
Tissue Cell. 2018 Oct;54:80-93. doi: 10.1016/j.tice.2018.08.006. Epub 2018 Aug 19.
Despite its wide range of application, cyclophosphamide (CP) exhibits a wide range of adverse effects including reproductive toxicity. The emerging field of zinc oxide nanoparticles (ZnO NPs) therapy may provide a new hope for prevention of CP induced gonadal toxicity. Herein, we aim to investigate the possible role of ZnO NPs as a new strategy to protect against CP induced testicular injury. Sixty adult male albino rats were divided into 3 groups; control, CP treated and CP + ZnO NPs treated groups. CP group was injected with CP (5 mg/kg/day), whereas CP + ZnO NPs group was concomitantly injected with CP and ZnO NPs (5 mg/kg/day). Testicular specimens were processed for histological, ultrastructural and c-kit immunohistochemical study. Biochemical analysis for tissue malondialdehyde and serum testosterone was done in addition to sperm morphology assay and cytogenetic study. Our results revealed that CP induced deleterious testicular histopathological, biochemical and genetic alterations that were effectively prevented by ZnO NPs.
尽管环磷酰胺（CP）应用广泛，但它会产生包括生殖毒性在内的多种不良反应。新兴的氧化锌纳米颗粒（ZnO NPs）治疗领域可能为预防CP诱导的性腺毒性带来新希望。在此，我们旨在研究ZnO NPs作为预防CP诱导睾丸损伤的新策略的潜在作用。将60只成年雄性白化病大鼠分为3组：对照组、CP治疗组和CP + ZnO NPs治疗组。CP组注射CP（5 mg/kg/天），而CP + ZnO NPs组同时注射CP和ZnO NPs（5 mg/kg/天）。对睾丸标本进行组织学、超微结构和c-kit免疫组织化学研究。除了精子形态学分析和细胞遗传学研究外，还对组织丙二醛和血清睾酮进行了生化分析。我们的结果显示，CP诱导了有害的睾丸组织病理学、生化和基因改变，而ZnO NPs有效地预防了这些改变。
