Instrumental Analysis Center, School of Pharmacy, Yancheng Teachers University, Yancheng 224007, China.
Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, China.
Food Chem. 2019 Jan 30;272:411-417. doi: 10.1016/j.foodchem.2018.08.073. Epub 2018 Aug 18.
The relatively new technique, desorption corona beam ionization (DCBI), was coupled with an ion-trap mass spectrometer for the rapid detection of 10 antimicrobials in animal-derived food. Under positive ion mode, 10 common antimicrobials were identified without prior sample preparation. With simple pre-treatment, semi-quantification based on peak area was achieved. There was a good correlation (R > 0.99) in the rational linear ranges (1-3 orders of magnitude) for all 10 target analytes. Compared with a conventional liquid chromatographic method, the DCBI-MS method was highly sensitive; e.g., roxithromycin was detected at 0.02 µg/g, whereas the value was 0.2 mg/kg in the European Union Commission Regulation (No 37/2010) and 0.3 μg/mL in a recently reported LC method. The proposed method allowed for the simple, rapid, sensitive and specific detection of antimicrobials in animal-derived foods. Our approach could be used for other atmospheric pressure chemical ionization (APCI) related ambient mass spectrometry methods to detect other compounds.
相对较新的技术，解吸电晕束电离（DCBI），与离子阱质谱仪联用，用于快速检测动物源性食品中的 10 种抗生素。在正离子模式下，无需样品制备即可鉴定 10 种常见抗生素。经过简单的预处理，基于峰面积实现了半定量。对于所有 10 种目标分析物，在合理的线性范围内（1-3 数量级）都有很好的相关性（R>0.99）。与传统的液相色谱法相比，DCBI-MS 方法具有很高的灵敏度；例如，罗红霉素的检测限为 0.02 µg/g，而欧盟委员会条例（No 37/2010）规定的检测限为 0.2 mg/kg，最近报道的 LC 方法的检测限为 0.3 µg/mL。该方法允许在动物源性食品中简单、快速、灵敏和特异性地检测抗生素。我们的方法可用于其他大气压化学电离（APCI）相关的环境质谱方法来检测其他化合物。
