Hou Yu-Lan, Wu Shuang, Wang Hua, Zhao Yong, Liao Peng, Tian Qing-Qing, Sun Wen-Jian, Chen Bo
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
Yao Xue Xue Bao. 2013 Jan;48(1):113-8.
A novel rapid method for detection of the illicit beta2-agonist additives in health foods and traditional Chinese patent medicines was developed with the desorption corona beam ionization mass spectrometry (DCBI-MS) technique. The DCBI conditions including temperature and sample volume were optimized according to the resulting mass spectra intensity. Matrix effect on 9 beta2-agonists additives was not significant in the proposed rapid determination procedure. All of the 9 target molecules were detected within 1 min. Quantification was achieved based on the typical fragment ion in MS2 spectra of each analyte. The method showed good linear coefficients in the range of 1-100 mg x L(-1) for all analytes. The relative deviation values were between 14.29% and 25.13%. Ten claimed antitussive and antiasthmatic health foods and traditional Chinese patent medicines from local pharmacies were analyzed. All of them were negative with the proposed DCBI-MS method. Without tedious sample pretreatments, the developed DCBI-MS is simple, rapid and sensitive for rapid qualification and semi-quantification of the illicit beta2-agonist additives in health foods and traditional Chinese patent medicines.
采用解吸电晕束电离质谱（DCBI-MS）技术，开发了一种用于检测保健食品和中成药中非法β2-激动剂添加剂的新型快速方法。根据所得质谱强度，对包括温度和样品体积在内的DCBI条件进行了优化。在所提出的快速测定方法中，基质对9种β2-激动剂添加剂的影响不显著。所有9种目标分子在1分钟内均可检测到。基于各分析物MS2谱中的典型碎片离子进行定量。该方法对所有分析物在1-100 mg·L(-1)范围内均显示出良好的线性系数。相对偏差值在14.29%至25.13%之间。对当地药店声称具有止咳平喘功效的10种保健食品和中成药进行了分析。采用所提出的DCBI-MS方法检测，结果均为阴性。所开发的DCBI-MS无需繁琐的样品预处理，对于快速鉴定和半定量保健食品和中成药中的非法β2-激动剂添加剂而言，具有简单、快速和灵敏的特点。
