College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China; Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China; Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China.
Food Chem. 2019 Jan 30;272:586-595. doi: 10.1016/j.foodchem.2018.08.017. Epub 2018 Aug 4.
β-Agarase was immobilized by using tannic acid modified-FeO magnetic nanoparticles (TA-MNPs) as a supportmaterial. The MNPs were synthesized by improved chemical coprecipitation method and modified with TA for agarase immobilization. TA-MNPs and immobilized β-agarase were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA), all of which indicated the successful surface modification of MNPs with TA and the immobilization of β-agarase. The optimal immobilization conditions for 25 mg TA-MNPs included 100 r/min oscillation speed, immobilization time of 2 h, immobilization temperature of 15 °C, and initial β-agarase concentration of 3 mL (480 U). Immobilized β-agarase showed better pH and thermal stability and excellent reusability than the free enzyme. Results revealed the promising application of β-agarase-TA-MNPs for the preparation of neoagaro-oligosaccharides with different averagepolymerizationdegrees and varying activities in the antioxidant.
β-琼胶酶通过使用单宁酸修饰的 FeO 磁性纳米粒子（TA-MNPs）作为固定化载体材料进行固定化。MNPs 通过改进的化学共沉淀法合成，并通过 TA 进行修饰以用于琼脂酶固定化。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和热重分析（TGA）对 TA-MNPs 和固定化的β-琼胶酶进行了表征，所有这些都表明 MNPs 成功地用 TA 进行了表面修饰，并且β-琼胶酶得到了固定化。对于 25mg TA-MNPs，最佳的固定化条件包括 100r/min 的振荡速度、2h 的固定化时间、15°C 的固定化温度和 3mL（480U）初始β-琼胶酶浓度。固定化的β-琼胶酶显示出比游离酶更好的 pH 值和热稳定性以及出色的可重复使用性。结果表明，β-琼胶酶-TA-MNPs 在制备具有不同平均聚合度和不同抗氧化活性的 neoagaro-寡糖方面具有广阔的应用前景。
