Rehabilitation Research Chair, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Clin Nutr. 2019 Oct;38(5):2231-2238. doi: 10.1016/j.clnu.2018.09.024. Epub 2018 Sep 25.
Circulating microRNAs (miRNAs) as valuable biomarkers yielded important insights into the pathogenesis of obesity.
This study aimed to describe the circulating miRNA profile for adolescences and its association with the circulating levels leptin and adiponectin according to specific degree of obesity.
RT-PCR and immunoassy analysis were used to study circulating miRNA profile, adipokines; adiponectin (A), leptin (L), and L/A ratio as well as other factors of metabolic syndrome (MS) in 250 adolescents with severe obesity.
In morbidly obese adolescents, we identified at least 10 circulating miRNAs, including increased concentrations of miRNAS; miR-142-3p, miR-140-5p, miR-222 miR-143, miR-130, and decreased concentrations of miR-532-5p, miR-423-5p, miR-520c-3p, miR-146a, and miR-15a, which were strongly linked to measures of BMI, WHtR, adipokines; adiponectin, leptin, L/A ratio, and other MS related biomarkers such as FBS, insulin, HOMA-IR, C-peptide, and circulated plasma lipids such as TG, HDL-C, and LDL-C.
Circulating miRNAs showed significant association with plasma levels of adipokines; adiponectin, leptin, and L/A ratios in adolescents with severe obesity. The study provides that regulation of miRNAs expression is associated with adipokines, and other related MS metabolic factors. Thus, early detection of any changes in circulating miRNAs profiles may play a promising role in identifying obese children or adolescents who may suffer from severe metabolic syndrome.
循环 microRNAs（miRNAs）作为有价值的生物标志物，为肥胖症的发病机制提供了重要的见解。
本研究旨在描述青少年的循环 miRNA 谱，并根据肥胖的特定程度描述其与循环瘦素和脂联素水平的关系。
使用 RT-PCR 和免疫分析来研究 250 名严重肥胖青少年的循环 miRNA 谱、脂联素（A）、瘦素（L）和 L/A 比值以及代谢综合征（MS）的其他因素。
在病态肥胖的青少年中，我们确定了至少 10 种循环 miRNA，包括 miR-142-3p、miR-140-5p、miR-222、miR-143、miR-130 的浓度增加，miR-532-5p、miR-423-5p、miR-520c-3p、miR-146a 和 miR-15a 的浓度降低，这些 miRNA 与 BMI、WHtR、脂联素、瘦素、L/A 比值和其他 MS 相关生物标志物如 FBS、胰岛素、HOMA-IR、C 肽和循环血浆脂质如 TG、HDL-C 和 LDL-C 密切相关。
循环 miRNA 与严重肥胖青少年血浆中脂联素、瘦素和 L/A 比值呈显著相关。该研究表明，miRNA 表达的调节与脂联素和其他相关 MS 代谢因素有关。因此，循环 miRNA 谱的任何变化的早期检测可能在识别可能患有严重代谢综合征的肥胖儿童或青少年方面发挥有希望的作用。
