Pulbutr Pawitra, Rattanakiat Sakulrat, Phetsaardeiam Natnicha, Modtaku Patcharapa, Denchai Rattazart, Jaruchotikamol Achida, Khunawattanakul Wanwisa
Pak J Biol Sci. 2018 Jan;21(6):300-306. doi: 10.3923/pjbs.2018.300.306.
Streptococcus mutans is a dominant causative pathogen of dental caries, which is a major oral health problem affecting million people worldwide. Derris reticulata is a medicinal plant possessing antimicrobial activity against several Gram-positive pathogenic bacteria. None the less, its effects on growth and cariogenic properties of S. mutans has not been clearly established. This study aimed to investigate the antibacterial and anti cariogenic activities of the D. reticulata ethanolic stem extract.
The TLC analysis was performed to authenticate the D. reticulata sample. Minimum inhibition concentration and minimum bactericidal concentration were determined by using broth dilution and drop plate methods, respectively. Sucrose dependent and sucrose independent-adherences, biofilm formation and glycolytic pH drop assays were performed to evaluate the anticariogenic activity.
The ethanolic stem extract of D. reticulata possessed the antibacterial activity against S. mutans with the MIC and MBC of 0.875±0.250 and 1.750±0.500 mg mL-1, respectively. The extract at the lower concentrations of sub-MIC also had significant inhibitory actions against the cariogenic properties of S. mutans, including surface adherence, biofilm formation and glycolytic acid production.
The D. reticulata stem extract had a substantial anticariogenic activities and thus potentially be developed as an oral health care product for dental caries prevention in the near future.
变形链球菌是龋齿的主要致病病原体，龋齿是影响全球数百万人的主要口腔健康问题。毛鱼藤是一种对多种革兰氏阳性病原菌具有抗菌活性的药用植物。然而，其对变形链球菌生长和致龋特性的影响尚未明确。本研究旨在探讨毛鱼藤乙醇提取物的抗菌和抗龋活性。
采用薄层色谱分析对毛鱼藤样品进行鉴定。分别采用肉汤稀释法和点滴平板法测定最低抑菌浓度和最低杀菌浓度。通过蔗糖依赖性和非依赖性黏附、生物膜形成及糖酵解pH值下降试验评估抗龋活性。
毛鱼藤乙醇提取物对变形链球菌具有抗菌活性，其最低抑菌浓度和最低杀菌浓度分别为0.875±0.250和1.750±0.500 mg/mL。低于最低抑菌浓度的提取物对变形链球菌的致龋特性也有显著抑制作用，包括表面黏附、生物膜形成和糖酵解产酸。
毛鱼藤茎提取物具有显著的抗龋活性，因此在不久的将来有可能被开发为预防龋齿的口腔保健产品。
