体外肉桂水提取物对尼古丁诱导的变形链球菌生物膜的抗龋作用。
In vitro Cariostatic effects of cinnamon water extract on nicotine-induced Streptococcus mutans biofilm.
机构信息
Department of Restorative Dental Sciences, College of Dentistry, King Khalid University, Abha, Saudi Arabia.
Department of Cariology, Operative Dentistry and Dental Public Health - Cariology and operative dentistry, Indiana University School of Dentistry, Indianapolis, IN, 46202, USA.
出版信息
BMC Complement Med Ther. 2020 Feb 11;20(1):45. doi: 10.1186/s12906-020-2840-x.
BACKGROUND
Dental caries is one of the most prevalent chronic oral diseases worldwide. Dental caries is mainly associated with Streptococcus mutans and the Lactobacillus species. A specific relationship was found between nicotine and S. mutans growth as the presence of nicotine increased S. mutans biofilm formation. Nicotine is able to increase the number of S. mutans and extracellular polysaccharide (EPS) synthesis. Among the widely used herbs and spices is cinnamon which demonstrated a strong antibacterial activity against a wide variety of bacteria including S. mutans and showed the ability to inhibit S. mutans biofilm formation. Cinnamon essential oil, obtained from the leaves of C. zeylanicum, has been demonstrated to be effective against S. mutans and Lactobacillus acidophilus, which are partially responsible for dental plaque formation and caries development. The aim of this study was to identify the effects of nicotine exposure on the inhibitory effects of cinnamon water extract on S. mutans biofilm formation.
MATERIALS AND METHODS
A 24-h culture of S. mutans UA159 in microtiter plates was treated with varying nicotine concentrations (0-32 mg/ml) in Tryptic Soy broth supplemented with 1% sucrose (TSBS) with or without a standardized concentration (2.5 mg/ml) of cinnamon water extract. A spectrophotometer was used to determine total growth absorbance and planktonic growth. The microtiter plate wells were washed, fixed and stained with crystal violet dye and the absorbance measured to determine biofilm formation.
RESULTS
The presence of 2.5 mg/ml cinnamon water extract inhibits nicotine-induced S. mutans biofilm formation from 34 to 98% at different concentrations of nicotine (0-32 mg/ml).
CONCLUSION
The results demonstrated nicotine-induced S. mutans biofilm formation is decreased from 34 to 98% in the presence of 2.5 mg/ml cinnamon water extract. This provides further evidence about the biofilm inhibitory properties of cinnamon water extract and reconfirms the harmful effects of nicotine.
背景
龋齿是全球最普遍的慢性口腔疾病之一。龋齿主要与变形链球菌和乳杆菌属有关。研究发现，尼古丁与变形链球菌的生长之间存在特定关系，因为尼古丁的存在会增加变形链球菌生物膜的形成。尼古丁能够增加变形链球菌的数量和细胞外多糖（EPS）的合成。肉桂是一种广泛使用的草药和香料，它对包括变形链球菌在内的多种细菌表现出强烈的抗菌活性，并显示出抑制变形链球菌生物膜形成的能力。从锡兰肉桂的叶子中提取的肉桂精油已被证明对变形链球菌和嗜酸乳杆菌有效，这两种细菌部分负责牙菌斑的形成和龋齿的发展。本研究旨在确定尼古丁暴露对肉桂水提取物抑制变形链球菌生物膜形成的影响。
材料和方法
在含有 1%蔗糖的胰蛋白胨大豆肉汤（TSBS）中，用微量滴定板培养 24 小时的变形链球菌 UA159，用不同浓度的尼古丁（0-32mg/ml）处理，并加入或不加入标准化浓度（2.5mg/ml）的肉桂水提取物。用分光光度计测定总生长吸光度和浮游生长。用微量滴定板孔冲洗、固定和用结晶紫染料染色，然后测定吸光度以确定生物膜形成。
结果
在不同浓度的尼古丁（0-32mg/ml）存在下，2.5mg/ml 肉桂水提取物抑制尼古丁诱导的变形链球菌生物膜形成，抑制率为 34%至 98%。
结论
结果表明，在 2.5mg/ml 肉桂水提取物存在下，尼古丁诱导的变形链球菌生物膜形成减少了 34%至 98%。这进一步证明了肉桂水提取物的生物膜抑制特性，并再次证实了尼古丁的有害作用。
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