Edwards S W
J Clin Lab Immunol. 1987 Jan;22(1):35-9.
The role of myeloperoxidase in luminol- and lucigenin-dependent chemiluminescence of stimulated human neutrophils has been investigated using purified myeloperoxidase and anti-(human myeloperoxidase) antiserum. This antiserum has been used as a specific enzyme inhibitor to assess myeloperoxidase-dependent neutrophil functions in single preparations of cells, thus overcoming the limitations inherent in other approaches using non-specific haem-inhibitors or myeloperoxidase-deficient neutrophils. The results show that luminol-dependent chemiluminescence is largely dependent on both oxidase activity and degranulation (of myeloperoxidase), while lucigenin monitors oxidase activity independently of the extent of degranulation. Since oxidase activation can occur in the absence of degranulation, assays utilizing luminol-dependent chemiluminescence to measure oxidant generation by stimulated neutrophils should include saturating levels of exogenous myeloperoxidase to overcome this problem.
利用纯化的髓过氧化物酶和抗（人髓过氧化物酶）抗血清，研究了髓过氧化物酶在受刺激的人中性粒细胞的鲁米诺和光泽精依赖性化学发光中的作用。该抗血清已被用作一种特异性酶抑制剂，以评估细胞单一制剂中髓过氧化物酶依赖性中性粒细胞功能，从而克服了使用非特异性血红素抑制剂或髓过氧化物酶缺陷中性粒细胞的其他方法所固有的局限性。结果表明，鲁米诺依赖性化学发光在很大程度上依赖于氧化酶活性和（髓过氧化物酶的）脱颗粒，而光泽精独立于脱颗粒程度监测氧化酶活性。由于氧化酶激活可在无脱颗粒的情况下发生，利用鲁米诺依赖性化学发光来测量受刺激中性粒细胞产生氧化剂的检测应包括饱和水平的外源性髓过氧化物酶以克服此问题。
