Cerqueira Juliana Aparecida, Restan Wilmer Alejandro Zamora, Fonseca Mayara Gonçalves, Catananti Lucas Azevedo, de Almeida Maria Luiza Mendes, Junior Walter Heinz Feringer, Pereira Gener Tadeu, Carciofi Aulus Cavalieri, de Camargo Ferraz Guilherme
São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences Jaboticabal, São Paulo, Brazil, 14884-900.
São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences Jaboticabal, São Paulo, Brazil, 14884-900..
Res Vet Sci. 2018 Dec;121:31-39. doi: 10.1016/j.rvsc.2018.10.004. Epub 2018 Oct 3.
Few data exist on the physiological consequences of an incremental exercise test (IET) and training in dogs. Here we evaluated the effect of an endurance-training program (ETP) on the kinetics of the serum biomarkers: creatine kinase (CK), aspartate aminotransferase (AST), myoglobin, cardiac troponin I (cTnI), and creatine kinase isoenzyme MB (CK-MB). Eighteen Beagle dogs were assigned to three groups: sedentary (S), untrained (U), and trained (T). The dogs from the groups T and U underwent IET, and their lactate threshold (LT) was determined. The LT and velocity corresponding to the LT (VLT) was determined by visual inspection. The VLT was utilized to determine the training intensity level. Thus, the dogs from group T underwent an eight-week aerobic conditioning program with the intensity training set to 70-80% of the VLT. Next, dogs from the groups T and U have submitted to IET again. The group S did not undergo IETs or training. The maximal velocity (Vmax) at which the dog achieves in the IET and heart rate were determined. For biomarkers, venous blood samples were collected before the experimental procedure (baseline) and before and 1, 6, 12, 24, 48, and 72 h after the IETs. VLT and Vmax increased in group T (P < .05), indicating an improvement in dogs' aerobic and anaerobic fitness. CK and AST rose (P < .05), peaking 6 h after both IETs and returning to baseline levels after 12-24 h. Levels of cTnI and myoglobin were unaltered. CK-MB peaked 1 h after the IETs and returned to baseline levels after 12 h (P < .05). We concluded that ETP improved the aerobic capacity of the dogs without any skeletal or cardiac muscle injury.
关于犬类递增运动试验（IET）及训练的生理影响，现有数据较少。在此，我们评估了耐力训练计划（ETP）对血清生物标志物动力学的影响：肌酸激酶（CK）、天冬氨酸转氨酶（AST）、肌红蛋白、心肌肌钙蛋白I（cTnI）和肌酸激酶同工酶MB（CK-MB）。18只比格犬被分为三组：久坐组（S）、未训练组（U）和训练组（T）。T组和U组的犬进行IET，并测定其乳酸阈值（LT）。通过目视检查确定LT及对应于LT的速度（VLT）。VLT用于确定训练强度水平。因此，T组的犬进行了为期八周的有氧调节计划，强度训练设定为VLT的70-80%。接下来，T组和U组的犬再次进行IET。S组未进行IET或训练。测定犬在IET中达到的最大速度（Vmax）和心率。对于生物标志物，在实验程序前（基线）以及IET前和IET后1、6、12、24、48和72小时采集静脉血样。T组的VLT和Vmax增加（P<0.05），表明犬的有氧和无氧适应性有所改善。CK和AST升高（P<0.05），在两次IET后6小时达到峰值，并在12-24小时后恢复到基线水平。cTnI和肌红蛋白水平未改变。CK-MB在IET后1小时达到峰值，并在12小时后恢复到基线水平（P<0.05）。我们得出结论，ETP提高了犬的有氧能力，且未造成任何骨骼肌或心肌损伤。
