Buchta R, Fridkin M, Pontet M, Romeo D
Peptides. 1986 Nov-Dec;7(6):961-8. doi: 10.1016/0196-9781(86)90121-x.
Peptides containing Lys-Pro-Arg or Thr-Lys-Arg segments corresponding to various regions of human C-reactive protein were synthesized. The peptides prepared were composed of amino acid residues, 37-58, 51-58, 173-187 and 181-187 of C-reactive protein. The relationship between C-reactive protein, its synthetic fragments and tuftsin (Thr-Lys-Pro-Arg) was investigated in binding studies, enhancement of phagocytosis and change in cyclic nucleotide levels of mouse macrophages. The peptides AA 51-58 and 181-187 did enhance macrophage phagocytosis capacity to a similar extent to that of tuftsin. They showed however only negligible binding to the cells. The effect of C-reactive protein and the synthetic peptides on metabolic activity of neutrophils was also investigated. It was shown that the peptides inhibited to some degree superoxide production, lysozyme release and Vitamin B12 binding protein release from neutrophils in the absence and presence of the stimulants, PMA or Con A. Comparable activity with tuftsin was not found.
合成了含有对应于人C反应蛋白不同区域的赖氨酸-脯氨酸-精氨酸或苏氨酸-赖氨酸-精氨酸片段的肽。所制备的肽由C反应蛋白的37 - 58、51 - 58、173 - 187和181 - 187位氨基酸残基组成。在结合研究、吞噬作用增强以及小鼠巨噬细胞环核苷酸水平变化方面，研究了C反应蛋白、其合成片段与促吞噬素（苏氨酸-赖氨酸-脯氨酸-精氨酸）之间的关系。肽AA 51 - 58和181 - 187确实在与促吞噬素相似的程度上增强了巨噬细胞的吞噬能力。然而，它们与细胞的结合作用微乎其微。还研究了C反应蛋白和合成肽对中性粒细胞代谢活性的影响。结果表明，在不存在和存在刺激物佛波酯或刀豆蛋白A的情况下，这些肽在一定程度上抑制了中性粒细胞中超氧化物的产生、溶菌酶的释放以及维生素B12结合蛋白的释放。未发现与促吞噬素具有相当活性的情况。
