Najjar V A, Konopinska D, Chaudhuri M K, Schmidt D E, Linehan L
Mol Cell Biochem. 1981 Dec 4;41:3-12.
Some of the properties of the tetrapeptide tuftsin, Thr-Lys-Pro-Arg, are discussed. We describe three phases of tuftsin activation of the macrophage. Tuftsinyltuftsin, the octapeptide Thr-Lys-Pro-Arg-Thr-Lys-Pro-Arg, was synthesized with a view of minimizing the formation of Lys-Pro-Arg, from tuftsin by tissue aminopeptidases. The tripeptide is a tuftsin inhibitor. The octapeptide proved to be quite effective in prolonging the life of syngeneic mice injected with L1210 leukemia cells. Its effect in our laboratory, was considerably better than we could obtain with tuftsin. A simple method for purifying tuftsin by high performance liquid chromatography is described using 0.75% trifluoroacetic acid in water. The tuftsin sequence Thr-Lys-Pro-Arg is present in P12 protein of Rausher murine leukemia virus. A close analog Thr-Arg-Pro-Lys appears in yet another virus protein the haemagglutinin of influenza virus. A second close analog Thr-Arg-Pro-Arg forms the penultimate carboxyterminal of a pancreatic polypeptide found in human and several animals.
本文讨论了四肽促吞噬素（苏氨酸-赖氨酸-脯氨酸-精氨酸）的一些特性。我们描述了促吞噬素激活巨噬细胞的三个阶段。为了尽量减少组织氨肽酶从促吞噬素中生成赖氨酸-脯氨酸-精氨酸，合成了八肽促吞噬素（苏氨酸-赖氨酸-脯氨酸-精氨酸-苏氨酸-赖氨酸-脯氨酸-精氨酸）。三肽是促吞噬素抑制剂。八肽在延长注射L1210白血病细胞的同基因小鼠寿命方面被证明相当有效。在我们实验室，其效果比促吞噬素要好得多。本文介绍了一种使用0.75%三氟乙酸水溶液通过高效液相色谱法纯化促吞噬素的简单方法。促吞噬素序列苏氨酸-赖氨酸-脯氨酸-精氨酸存在于劳舍尔鼠白血病病毒的P12蛋白中。另一种密切类似物苏氨酸-精氨酸-脯氨酸-赖氨酸出现在另一种病毒蛋白——流感病毒的血凝素中。第二种密切类似物苏氨酸-精氨酸-脯氨酸-精氨酸构成了在人类和几种动物中发现的一种胰多肽的倒数第二个羧基末端。
