Voutilainen R, Miller W L
Proc Natl Acad Sci U S A. 1987 Mar;84(6):1590-4. doi: 10.1073/pnas.84.6.1590.
Insulin-like growth factors (IGFs) are single-chain polypeptides important for cell proliferation and growth. IGFs are produced in several tissues, suggesting that they function in a paracrine or autocrine fashion as well as functioning as endocrine hormones. We studied the hormonal regulation of IGF-I and IGF-II mRNA in human steroidogenic tissues. In cultured human ovarian granulosa cells, follicle-stimulating hormone, human chorionic gonadotropin, and dibutyryl cAMP increased IGF-II mRNA, but corticotropin [adrenocorticotropic hormone (ACTH)], chorionic somatomammotropin, growth hormone, prolactin, dexamethasone, estradiol, and progesterone had no effect. In cultured human fetal adrenal cells, ACTH and dibutyryl cAMP increased IGF-II mRNA accumulation, but human chorionic gonadotropin and angiotensin II did not. The same five size species of IGF-II mRNA were detected in transfer blots of RNA from granulosa cells and fetal adrenal cells, and all of these increased after hormonal stimuli. Dibutyryl cAMP also increased IGF-II mRNA accumulation in cultured human placental cells. Accumulation of mRNA for the cholesterol side-chain-cleavage monooxygenase [P450scc [corrected]; cholesterol, reduced-adrenal-ferredoxin:oxygen oxidoreductase (side-chain-cleaving), EC 1.14.15.6] was regulated in parallel with IGF-II mRNA in all these steroidogenic tissues. IGF-I mRNA was not detected in transfer blots of these RNAs, and the minimal amounts detected in dot blots showed no detectable change after any of the hormonal stimuli studied. The data indicate that the IGF-II gene is expressed in human steroidogenic tissues and is regulated by cAMP. These data suggest that IGF-II may act in an autocrine or paracrine fashion to stimulate the adrenal and gonadal growth stimulated by ACTH and gonadotropins, respectively.
胰岛素样生长因子（IGFs）是对细胞增殖和生长至关重要的单链多肽。IGFs在多个组织中产生，这表明它们以旁分泌或自分泌方式发挥作用，同时也作为内分泌激素发挥功能。我们研究了人类类固醇生成组织中IGF-I和IGF-II mRNA的激素调节。在培养的人卵巢颗粒细胞中，促卵泡激素、人绒毛膜促性腺激素和二丁酰环磷腺苷（dibutyryl cAMP）可增加IGF-II mRNA，但促肾上腺皮质激素[促肾上腺皮质激素（ACTH）]、绒毛膜促生长催乳素、生长激素、催乳素、地塞米松、雌二醇和孕酮则无作用。在培养的人胎儿肾上腺细胞中，ACTH和二丁酰环磷腺苷可增加IGF-II mRNA的积累，但人绒毛膜促性腺激素和血管紧张素II则无此作用。在颗粒细胞和胎儿肾上腺细胞的RNA转移印迹中检测到相同的5种大小的IGF-II mRNA，并且在激素刺激后所有这些mRNA均增加。二丁酰环磷腺苷也可增加培养的人胎盘细胞中IGF-II mRNA的积累。在所有这些类固醇生成组织中，胆固醇侧链裂解单加氧酶[P450scc[校正后]；胆固醇，还原型肾上腺铁氧化还原蛋白：氧氧化还原酶（侧链裂解），EC 1.14.15.6]的mRNA积累与IGF-II mRNA平行调节。在这些RNA的转移印迹中未检测到IGF-I mRNA，并且在斑点印迹中检测到的微量在任何所研究的激素刺激后均未显示出可检测到的变化。数据表明IGF-II基因在人类类固醇生成组织中表达并受环磷腺苷调节。这些数据表明IGF-II可能分别以自分泌或旁分泌方式作用，以刺激由ACTH和促性腺激素分别刺激的肾上腺和性腺生长。
