Tutun B, Elbe H, Vardi N, Parlakpinar H, Polat A, Gunaltili M, Guclu M M, Yasar E N
a Medical Faculty , Inonu University , Malatya , Turkey.
b Medical Faculty, Department of Histology and Embryology , Mugla Sıtkı Kocman University , Mugla , Turkey.
Biotech Histochem. 2019 Feb;94(2):84-91. doi: 10.1080/10520295.2018.1508746. Epub 2018 Oct 15.
Hyperglycemia increases reactive oxygen species (ROS) and the resulting oxidative stress contributes to the development of diabetic complications. Dexpanthenol (Dxp) is the biological active form of pantothenic acid. We investigated whether Dxp administration could decrease oxidative stress as a way to treat renal complications of diabetes mellitus (DM). Thirty-two male Wistar albino rats were divided into four groups: control, Dxp, DM and DM + Dxp. Experimental diabetes was induced by a single dose of streptozotocin (STZ). After administration of STZ, the DM + Dxp group was administered 500 mg/kg Dxp intraperitoneally every day for 6 weeks. At the end of the study, blood glucose levels were measured and rats were sacrificed. Kidneys were embedded in paraffin, sectioned and stained with hematoxylin and eosin, and periodic acid-Schiff. The mean malondialdehyde levels, glutathione peroxidase, superoxide dismutase and catalase activities, and total antioxidant and total oxidant status also were measured. The control group was normal in histological appearance. We observed congestion, inflammation, glomerulosclerosis, tubular desquamation, loss of villi and hydropic degeneration in tubule cells in the DM group. Indicators of oxidative stress were elevated and antioxidant activity was reduced in the DM group compared to controls. In the DM + Dxp group, oxidative stress was decreased, antioxidant activity was increased and histopathological changes were reduced compared to the DM group. We found that Dxp exhibited ameliorative effects on STZ induced diabetic nephropathy by increasing antioxidant activity.
高血糖会增加活性氧（ROS），由此产生的氧化应激会促使糖尿病并发症的发展。右泛醇（Dxp）是泛酸的生物活性形式。我们研究了给予Dxp是否可以通过降低氧化应激来治疗糖尿病（DM）的肾脏并发症。将32只雄性Wistar白化大鼠分为四组：对照组、Dxp组、DM组和DM + Dxp组。通过单剂量链脲佐菌素（STZ）诱导实验性糖尿病。给予STZ后，DM + Dxp组每天腹腔注射500 mg/kg Dxp，持续6周。在研究结束时，测量血糖水平并处死大鼠。将肾脏嵌入石蜡，切片并用苏木精和伊红以及过碘酸-希夫染色。还测量了丙二醛平均水平、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶活性以及总抗氧化和总氧化状态。对照组组织学外观正常。我们在DM组中观察到肾小管细胞出现充血、炎症、肾小球硬化、肾小管脱屑、绒毛丧失和水样变性。与对照组相比，DM组的氧化应激指标升高，抗氧化活性降低。与DM组相比，DM + Dxp组的氧化应激降低，抗氧化活性增加，组织病理学变化减少。我们发现Dxp通过增加抗氧化活性对STZ诱导的糖尿病肾病具有改善作用。
