Hashemi Seyede Sahar, Najari Mahmoud, Parvin Milad, Kalani Mohammad Mehdi, Assadi Majid, Seyedian Ramin, Zaeri Sasan
Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran.
Department of Oral & Maxillofacial Surgery, School of Dentistry, Bushehr University of Medical Sciences, Bushehr, Iran.
Iran J Basic Med Sci. 2024;27(1):97-106. doi: 10.22038/IJBMS.2023.71412.15526.
Knowing the detrimental role of oxidative stress in wound healing and the anti-oxidant properties of Dexpanthenol (Dex), we aimed to produce Dex-loaded electrospun core/shell nanofibers for wound healing study. The novelty was measuring oxidative stress in wounds to know how oxidative stress was affected by Dex-loaded fibers.
TPVA solution containing Dex 6% (w/v) (core) and PVA/chitosan solution (shell) were coaxially electrospun with variable injection rates of the shell solution. Fibers were then tested for physicochemical properties, drug release profile, and effects on wound healing. Levels of tissue lipid peroxidation and superoxide dismutase activity were measured.
Fibers produced at shell injection rate of 0.3 ml/hr (F3 fibers) showed core/shell structure with an average diameter of 252 nm, high hydrophilicity (swelling: 157% at equilibrium), and low weight loss (13.6%). Dex release from F3 fibers seemed to be ruled by the Fickian mechanism based on the Korsmeyer-Peppas model (R = 0.94, n = 0.37). Dex-loaded F3 fibers promoted fibroblast viability (128.4%) significantly on day 5 and also accelerated wound healing compared to the neat F3 fibers at macroscopic and microscopic levels on day 14 post-wounding. The important finding was a significant decrease in malondialdehyde (0.39 nmol/ mg protein) level and an increase in superoxide dismutase (5.29 unit/mg protein) activity in Dex-loaded F3 fiber-treated wound tissues.
Dex-loaded core/shell fibers provided nano-scale scaffolds with sustained release profile that significantly lowered tissue oxidative stress. This finding pointed to the importance of lowering oxidative stress to achieve proper wound healing.
鉴于了解氧化应激在伤口愈合中的有害作用以及右泛醇（Dex）的抗氧化特性，我们旨在制备负载Dex的电纺核/壳纳米纤维用于伤口愈合研究。新颖之处在于测量伤口中的氧化应激，以了解负载Dex的纤维如何影响氧化应激。
将含有6%（w/v）Dex的TPVA溶液（核）和PVA/壳聚糖溶液（壳）以不同的壳溶液注射速率进行同轴电纺。然后对纤维进行物理化学性质、药物释放曲线以及对伤口愈合影响的测试。测量组织脂质过氧化水平和超氧化物歧化酶活性。
以0.3 ml/hr的壳注射速率制备的纤维（F3纤维）呈现核/壳结构，平均直径为252 nm，具有高亲水性（平衡时肿胀率：157%）和低重量损失（13.6%）。基于Korsmeyer-Peppas模型，F3纤维中Dex的释放似乎受菲克扩散机制控制（R = 0.94，n = 0.37）。负载Dex的F3纤维在第5天显著促进了成纤维细胞活力（128.4%），并且在伤口受伤后第14天，与纯F3纤维相比，在宏观和微观水平上均加速了伤口愈合。重要发现是，在负载Dex的F3纤维处理的伤口组织中，丙二醛水平显著降低（0.39 nmol/mg蛋白质），超氧化物歧化酶活性增加（5.29单位/mg蛋白质）。
负载Dex的核/壳纤维提供了具有缓释特性的纳米级支架，可显著降低组织氧化应激。这一发现表明降低氧化应激对于实现适当的伤口愈合很重要。
