Qin You, Chen Lin, Jin Jian, Huang Jianhua, Li Jiayu, Zhou Qingyijun, Zhou Rongrong, Zhang Shui-Han
Hunan University of Chinese Medicine, Changsha, P.R. China; Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, P.R. China.
Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, P.R. China; 2011 Collaboration and Innovation Center for Digital Chinese Medicine in Hunan, Changsha, P.R. China.
Int J Med Mushrooms. 2018;20(9):887-899. doi: 10.1615/IntJMedMushrooms.2018027390.
Ophiocordyceps xuefengensis has recently been identified as a sister taxon of O. sinensis and has long been recognized as a folk tonic, food, or invigorant in South China. This study had 3 aims: First, we analyzed the antioxidant activities of 3 kinds of caterpillar fungi; the antioxidative ability of the various caterpillar fungi were in the order of O. xuefengensis > Cordyceps militaris > O. sinensis. Second, we further identified the antioxidant compounds in O. xuefengensis by using various methods: multiwavelength DPPH high-performance liquid chromatography with diode array detection and quadrupole time-of-flight mass spectrometry. In this way, we found and identified 30 compounds that possessed potential antioxidant activity. Among these compounds, 23 compounds were determined to be composed of nucleosides, nucleotides, and amino acids. To our knowledge, several compounds in O. xuefengensis are reported here for the first time. Third, we compared the antioxidant markers in 3 caterpillar fungi. Results showed that the caterpillar fungi share some common antioxidants, while each also had its own characteristic compounds that correspond to its antioxidant capacity. This is to our knowledge the first report of the screening and identification of antioxidants in O. xuefengensis, and these results might provide beneficial contributions to understandings of O. xuefengensis on the basis of its chemical compounds and antioxidant activity.
雪峰虫草最近被鉴定为中华虫草的姐妹分类群，长期以来在中国南方一直被视为民间滋补品、食品或强身健体之物。本研究有3个目的：第一，我们分析了3种虫草的抗氧化活性；各种虫草的抗氧化能力顺序为雪峰虫草>蛹虫草>中华虫草。第二，我们通过多种方法进一步鉴定了雪峰虫草中的抗氧化化合物：采用多波长DPPH高效液相色谱-二极管阵列检测和四极杆飞行时间质谱法。通过这种方式，我们发现并鉴定了30种具有潜在抗氧化活性的化合物。在这些化合物中，有23种化合物被确定由核苷、核苷酸和氨基酸组成。据我们所知，雪峰虫草中的几种化合物在此首次被报道。第三，我们比较了3种虫草中的抗氧化标志物。结果表明，这3种虫草有一些共同的抗氧化剂，同时每种虫草也有其自身与抗氧化能力相对应的特征性化合物。据我们所知，这是首次对雪峰虫草中的抗氧化剂进行筛选和鉴定的报告，这些结果可能有助于从其化合物和抗氧化活性方面了解雪峰虫草。
