Zou Juan, Zhang Yating, Luo Yan, Fu Miaohua, Sun Beilin, Liu Shenggui
Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua 418000, China.
Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China.
Biology (Basel). 2024 Sep 2;13(9):686. doi: 10.3390/biology13090686.
Many species are used as traditional Chinese medicines. However, are entomopathogenic fungi in the family Clavicipitaceae of Ascomycota, and excessive harvesting severely disrupts natural habitat ecosystems. Artificial cultivation of fruiting bodies offers a viable strategy to protect the ecological environment and mitigate the depletion of wild resource. In this study, mononucleate hyphae were selected using DAPI fluorescence staining, the and strains of were successfully distinguished using loop-mediated isothermal amplification (LAMP). The chemical composition and bioactive components of fruiting bodies produced by these strains were compared. Results showed that the levels of adenosine, thymidine, adenine, guanosine, uridine, total amino acids, and total essential amino acids in the fruiting bodies of strains were 1.31 mg/g, 0.15 mg/g, 0.26 mg/g, 2.40 mg/g, 2.34 mg/g, 270.3 mg/g, and 102.5 mg/g, respectively, which were significantly higher than those in the sample. Contrastingly, the fruiting bodies of strains contained higher levels of mannose and polysaccharides, at 11.7% and 12.2%, respectively. The levels of toxic elements such as Al, Pb, As, and Hg in the fruiting bodies were 1.862 mg/kg, 0.0848 mg/kg, 0.534 mg/kg, and 0.0054 mg/kg, respectively, which were markedly lower than those in the fruiting bodies.
许多物种被用作传统中药。然而，它们属于子囊菌门麦角菌科的昆虫病原真菌，过度采收严重破坏了自然栖息地生态系统。人工栽培子实体为保护生态环境和缓解野生资源枯竭提供了一种可行的策略。在本研究中，使用DAPI荧光染色筛选单核菌丝，利用环介导等温扩增（LAMP）成功区分了它们的菌株。比较了这些菌株产生的子实体的化学成分和生物活性成分。结果表明，菌株子实体中腺苷、胸苷、腺嘌呤、鸟苷、尿苷、总氨基酸和总必需氨基酸的含量分别为1.31mg/g、0.15mg/g、0.26mg/g、2.40mg/g、2.34mg/g、270.3mg/g和102.5mg/g，显著高于样品中的含量。相反，菌株子实体中甘露糖和多糖的含量较高，分别为11.7%和12.2%。菌株子实体中铝、铅、砷和汞等有毒元素的含量分别为1.862mg/kg、0.0848mg/kg、0.534mg/kg和0.0054mg/kg，明显低于菌株子实体中的含量。
