Ouyang C, Huang T F
Toxicon. 1986;24(11-12):1099-106. doi: 10.1016/0041-0101(86)90136-4.
Among all the purified components from A. acutus venom, including ADPase, 5'-nucleotidase, phospholipase A2 and fibrinogenases, only the venom ADPase (50-100 micrograms/ml) shows marked inhibitory action on ADP (10 microM)-, collagen (10 micrograms/ml)- and sodium arachidonate (100 microM)-induced platelet aggregations of rabbit platelet-rich plasma. The venom 5'-nucleotidase (100 micrograms/ml) inhibited ADP-induced platelet aggregation by 31 +/- 4% (n = 4, P less than 0.05). Fibrinogenolytic enzymes (fractions I and IX, 100 micrograms/ml) did not significantly inhibit platelet aggregation induced by ADP (10 microM), collagen (10 micrograms/ml) or sodium arachidonate (100 microM). However, when the fibrinogenase (fraction IX, 100 micrograms/ml) was preincubated with platelet-rich plasma for 30 min it inhibited collagen (20 micrograms/ml)- and ADP (10 microM)-induced platelet aggregations by 34 +/- 9% (n = 4, P less than 0.05) and 35 +/- 6% (n = 4, P less than 0.05), respectively. The phospholipase A2 (100 micrograms/ml) did not affect platelet aggregation. The venom ADPase is a single chain polypeptide with a molecular weight of 94,000. The specific ADPase activity is estimated to be 4.3 mu moles Pi/min/mg of protein. It also possesses phosphodiesterase and weak 5'-nucleotidase activities.
在尖吻蝮蛇毒的所有纯化成分中，包括二磷酸腺苷酶（ADPase）、5'-核苷酸酶、磷脂酶A2和纤维蛋白原酶，只有蛇毒ADPase（50 - 100微克/毫升）对二磷酸腺苷（10微摩尔）、胶原（10微克/毫升）和花生四烯酸钠（100微摩尔）诱导的富含兔血小板血浆的血小板聚集有显著抑制作用。蛇毒5'-核苷酸酶（100微克/毫升）使二磷酸腺苷诱导的血小板聚集抑制31±4%（n = 4，P<0.05）。纤维蛋白溶解酶（组分I和IX，100微克/毫升）对二磷酸腺苷（10微摩尔）、胶原（10微克/毫升）或花生四烯酸钠（100微摩尔）诱导的血小板聚集没有显著抑制作用。然而，当纤维蛋白原酶（组分IX，100微克/毫升）与富含血小板血浆预孵育30分钟时，它分别使胶原（20微克/毫升）和二磷酸腺苷（10微摩尔）诱导的血小板聚集抑制34±9%（n = 4，P<0.05）和35±6%（n = 4，P<0.05）。磷脂酶A2（100微克/毫升）不影响血小板聚集。蛇毒ADPase是一种分子量为94,000的单链多肽。其特异性ADPase活性估计为4.3微摩尔无机磷/分钟/毫克蛋白质。它还具有磷酸二酯酶和较弱的5'-核苷酸酶活性。
