Reenstra W W, Bettencourt J D, Forte J G
Am J Physiol. 1987 Apr;252(4 Pt 1):G543-7. doi: 10.1152/ajpgi.1987.252.4.G543.
Net Cl- flux across the bullfrog gastric mucosa was examined to test the hypothesis that Cl-secretion (JClnet) can be driven by either of the two cation exchange pumps in the oxyntic cell. The effects on JClnet of ouabain, an Na+-K+ pump inhibitor, and omeprazole, an H+-K+ pump inhibitor were examined. Omeprazole abolished acid secretion (JH) and reduced JClnet in bullfrog gastric mucosa. For mucosae at open circuit the omeprazole-induced decrease in JH was not significantly different than the decrease in JClnet, and the transmucosal potential difference (PD) was increased. When short-circuited mucosae were treated with omeprazole, the decrease in JClnet was significantly less than the decrease in JH, and short-circuit current (SCC) was correspondingly increased. After treatment of short-circuited mucosae with ouabain, the omeprazole-induced decreases in JH and JClnet were not significantly different, and no change in SCC occurred. For open-circuited mucosae, pretreatment with ouabain resulted in a significantly smaller omeprazole-induced increase in the transmucosal PD than was seen without ouabain pretreatment. Our data 1) show that both the H+-K+ pump and the Na+-K+ pump can drive Cl- secretion and 2) suggest that inhibition of the H+-K+ pump with omeprazole stimulates the Na+-K+ pump.
检测了牛蛙胃黏膜的净氯离子通量，以验证壁细胞中的两种阳离子交换泵均可驱动氯离子分泌（JClnet）这一假说。研究了钠钾泵抑制剂哇巴因和氢钾泵抑制剂奥美拉唑对JClnet的影响。奥美拉唑消除了牛蛙胃黏膜的酸分泌（JH）并降低了JClnet。对于开路状态的黏膜，奥美拉唑引起的JH降低与JClnet降低无显著差异，且跨黏膜电位差（PD）升高。当用奥美拉唑处理短路黏膜时，JClnet的降低明显小于JH的降低，且短路电流（SCC）相应增加。用哇巴因处理短路黏膜后，奥美拉唑引起的JH和JClnet降低无显著差异，且SCC无变化。对于开路黏膜，与未用哇巴因预处理相比，用哇巴因预处理导致奥美拉唑引起的跨黏膜PD增加明显更小。我们的数据1）表明氢钾泵和钠钾泵均可驱动氯离子分泌，2）提示用奥美拉唑抑制氢钾泵可刺激钠钾泵。
