Reenstra W W, Forte J G
Am J Physiol. 1986 Jan;250(1 Pt 1):G76-84. doi: 10.1152/ajpgi.1986.250.1.G76.
Effects of thiocyanate (SCN-) and imidazole on acid secretion and Cl- flux across the isolated bullfrog gastric mucosa have been examined. For open-circuited mucosae, with Cl- Ringer on the nutrient side and 110 mM Na isethionate on the secretory side, the addition of 15 mM SCN- to either the secretory or nutrient side gave equivalent reductions in acid secretion (JH) and nutrient-to-secretory Cl- flux (JnsCl) . JnsSCN, the nutrient-to-secretory flux of SCN- (0.2 mu eq . cm-2 . h-1), was much less than the decrease in JH (1.88 mu eq . cm-2 . h-1). Addition of imidazole, phosphate, or histidine to the secretory side reversed SCN- inhibition of JnsCl. Addition of imidazole to the nutrient side gave equivalent restorations of JnsCl and JH. The increase in JnsSCN, 0.07 mu eq . cm-2 . h-1, was much less than the increase in either JH, 0.77, or JnsCl, 0.86 mu eq . cm-2 . h-1. Similar results were obtained for mucosae bathed with NaCl on the secretory side. With 15 mM SCN- on both sides, the flux ratio for Cl- was significantly larger (1.0) than the flux ratio for SCN- (0.47). The addition of nutrient imidazole increased the flux ratios for Cl- and SCN- to 1.29 and 1.16, respectively. Both SCN- and Cl- showed exchange diffusion. Inhibition of acid secretion with nutrient SCN- at short circuit also inhibited JnsCl but did not alter the partial conductance of Cl-.(ABSTRACT TRUNCATED AT 250 WORDS)
已研究了硫氰酸盐（SCN⁻）和咪唑对离体牛蛙胃黏膜酸分泌及氯离子跨膜通量的影响。对于开路黏膜，营养侧为含氯林格液，分泌侧为110 mM羟乙磺酸钠，在分泌侧或营养侧添加15 mM SCN⁻会使酸分泌（JH）和营养侧到分泌侧的氯离子通量（JnsCl）同等程度降低。SCN⁻从营养侧到分泌侧的通量（JnsSCN，0.2微当量·厘米⁻²·小时⁻¹）远低于JH的降低量（1.88微当量·厘米⁻²·小时⁻¹）。向分泌侧添加咪唑、磷酸盐或组氨酸可逆转SCN⁻对JnsCl的抑制。向营养侧添加咪唑可使JnsCl和JH同等程度恢复。JnsSCN增加了0.07微当量·厘米⁻²·小时⁻¹，远低于JH增加的0.77或JnsCl增加的0.86微当量·厘米⁻²·小时⁻¹。分泌侧用氯化钠灌注的黏膜也得到类似结果。两侧均含15 mM SCN⁻时，氯离子的通量比（1.0）显著大于SCN⁻的通量比（0.47）。向营养侧添加咪唑可使氯离子和SCN⁻的通量比分别增至1.29和1.16。SCN⁻和氯离子均表现出交换扩散。营养侧SCN⁻在短路时抑制酸分泌也会抑制JnsCl，但不改变氯离子的部分电导率。（摘要截短于250字）
