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A simple and rapid assay for heme attachment to apocytochrome c.

作者信息

Enosawa S, Ohashi A

出版信息

Anal Biochem. 1987 Jan;160(1):211-6. doi: 10.1016/0003-2697(87)90632-4.

DOI:10.1016/0003-2697(87)90632-4
PMID:3032018
Abstract

A method for assaying the covalent attachment of heme to apoprotein of cytochrome c was developed. 125I-labeled apoprotein was chemically prepared from 125I-labeled yeast cytochrome c (iso-1-cytochrome c). After incubation of 125I-apocytochrome c with yeast mitochondria, the product was extracted with Triton X-100, digested with trypsin in the presence or absence of a reducing agent, and then precipitated in trichloroacetic acid. The resulting precipitates were collected on nitrocellulose membranes and counted for radioactivity. The radioactivity correlated well with the appearance of a heme-containing peptide in the trypsin digested peptide fragments of cytochrome c. This procedure is simpler and faster than the previously reported methods.

摘要

相似文献

1
A simple and rapid assay for heme attachment to apocytochrome c.
Anal Biochem. 1987 Jan;160(1):211-6. doi: 10.1016/0003-2697(87)90632-4.
2
Localization of enzyme for heme attachment to apocytochrome c in yeast mitochondria.酵母线粒体中血红素与脱辅基细胞色素c结合所需酶的定位
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Import of cytochrome c into mitochondria: reduction of heme, mediated by NADH and flavin nucleotides, is obligatory for its covalent linkage to apocytochrome c.细胞色素c导入线粒体：由NADH和黄素核苷酸介导的血红素还原，是其与脱辅基细胞色素c共价连接所必需的。
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4340-4. doi: 10.1073/pnas.86.12.4340.
4
Formation of an iso-1-cytochrome c-like species containing a covalently bonded heme group from the apoprotein by a yeast cell-free system in the presence of hemin.在血红素存在的情况下，通过酵母无细胞系统由脱辅基蛋白形成含有共价结合血红素基团的异-1-细胞色素c样物种。
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On the specificity of cytochrome c synthetase in recognition of the amino acid sequence of apocytochrome c.
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Coupling of heme attachment to import of cytochrome c into yeast mitochondria. Studies with heme lyase-deficient mitochondria and altered apocytochromes c.血红素附着与细胞色素c导入酵母线粒体的偶联。对血红素裂解酶缺陷型线粒体和改变的脱辅基细胞色素c的研究。
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Role of cytochrome c heme lyase in mitochondrial import and accumulation of cytochrome c in Saccharomyces cerevisiae.细胞色素c血红素裂解酶在酿酒酵母线粒体细胞色素c导入和积累中的作用
Mol Cell Biol. 1991 Nov;11(11):5487-96. doi: 10.1128/mcb.11.11.5487-5496.1991.
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Formation of a cytochrome c-like species from horse apoprotein and hemin catalyzed by yeast mitochondrial cytochrome c synthetase.酵母线粒体细胞色素c合成酶催化马脱辅基蛋白和血红素形成细胞色素c样物质。
J Biol Chem. 1981 Aug 25;256(16):8646-51.
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Synthesis of a heme fragment of horse cytochrome c which forms a productive complex with a native apofragment.马细胞色素c的血红素片段的合成，该片段与天然脱辅基片段形成有效复合物。
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Reversible import of apocytochrome c into mitochondria.脱辅基细胞色素c可逆性导入线粒体。
Proc Natl Acad Sci U S A. 1990 Jul;87(13):4996-5000. doi: 10.1073/pnas.87.13.4996.

引用本文的文献

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The biosynthesis of bacterial and plastidic c-type cytochromes.细菌和质体 c 型细胞色素的生物合成。
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Cytochromes c biogenesis in a photosynthetic bacterium requires a periplasmic thioredoxin-like protein.光合细菌中细胞色素c的生物合成需要一种周质硫氧还蛋白样蛋白。
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