Cevallos-Cedeño Ramón E, Agulló Consuelo, Abad-Somovilla Antonio, Abad-Fuentes Antonio, Mercader Josep V
Institute of Agrochemistry and Food Technology (IATA), Spanish Council for Scientific Research (CSIC), Agustí Escardino 7, 46980 Paterna, Valencia, Spain.
Department of Organic Chemistry, University of Valencia, Doctor Moliner 50, 46100 Burjassot, Valencia, Spain.
ACS Omega. 2018 Sep 30;3(9):11950-11957. doi: 10.1021/acsomega.8b01784. Epub 2018 Sep 26.
Spirotetramat-a tetramic acid insecticide-is rapidly metabolized or degraded to give spirotetramat-enol; so, common residue definitions include the sum of both compounds. In the present study, two spirotetramat-functionalized derivatives (haptens) have been designed to generate immunoreagents to these molecules for rapid immunochemical analysis. Haptens have been synthesized with alternative linker tethering sites and, for the first time, high-affinity antibodies have been generated with different specificities to these active principles. Two sensitive assays have been developed using the same antibody in different formats, and by using linker-site heterologous haptens, the selectivity of the final immunoassay could be improved. A generic immunoassay with sensitivity similar to spirotetramat and spirotetramat-enol and a specific assay of spirotetramat-enol have been developed. The described antibody and bioconjugates showed great potential for sensitive immunosensor development and analysis of this complex analyte.
螺虫乙酯——一种四元酸杀虫剂——会迅速代谢或降解生成螺虫乙酯烯醇；因此，常见的残留定义包括这两种化合物的总和。在本研究中，设计了两种螺虫乙酯功能化衍生物（半抗原），以生成针对这些分子的免疫试剂，用于快速免疫化学分析。已合成具有不同连接位点的半抗原，并且首次生成了对这些活性成分具有不同特异性的高亲和力抗体。使用相同抗体以不同形式开发了两种灵敏的检测方法，并且通过使用连接位点异源半抗原，可以提高最终免疫检测的选择性。已开发出一种灵敏度与螺虫乙酯和螺虫乙酯烯醇相似的通用免疫检测方法以及一种螺虫乙酯烯醇的特异性检测方法。所描述的抗体和生物共轭物在灵敏免疫传感器的开发以及这种复杂分析物的分析方面显示出巨大潜力。
