Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwangju, Republic of Korea.
Department of Dental Hygiene, Chodang University, Muan, Republic of Korea.
Arch Physiol Biochem. 2020 Feb;126(1):74-81. doi: 10.1080/13813455.2018.1493607. Epub 2018 Oct 15.
This study evaluated the anti-inflammatory potential of a 40% prethanol extract of leaves (40% PeTP) using (RAW264.7 cells) and (carrageenan-induced inflammation model) experiments. Pretreatment with 40% PeTP significantly inhibited the LPS-induced expression of nitric oxide (NO), prostaglandin E (PGE), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines, including tumour necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in RAW264.7 cells, without inducing cytotoxicity. The inhibitory effects of 40% PeTP are mediated through suppression of the nuclear translocation of nuclear factor (NF)-κB and the phosphorylation of mitogen-activated protein kinases (MAPKs). Oral administration of 40% PeTP at 50, 100, and 200 mg/kg of body weight suppressed carrageenan-induced oedema in a dose-dependent manner. Collectively, our results suggested that 40% PeTP exerts potential anti-inflammatory effects by suppressing the activation of the NF-κB and MAPK pathways , and by reducing carrageenan-induced paw oedema .
本研究采用 RAW264.7 细胞和角叉菜胶诱导炎症模型实验，评估了 40%叶乙醇提取物（40%PeTP）的抗炎潜力。40%PeTP 预处理可显著抑制 LPS 诱导的 RAW264.7 细胞中一氧化氮（NO）、前列腺素 E（PGE）、诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）和炎症细胞因子，包括肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6 的表达，而无细胞毒性。40%PeTP 的抑制作用是通过抑制核因子（NF）-κB 的核转位和丝裂原活化蛋白激酶（MAPKs）的磷酸化来介导的。40%PeTP 以 50、100 和 200mg/kg 体重的剂量口服给药可剂量依赖性地抑制角叉菜胶诱导的水肿。综上所述，我们的研究结果表明，40%PeTP 通过抑制 NF-κB 和 MAPK 通路的激活以及减轻角叉菜胶诱导的爪肿胀来发挥潜在的抗炎作用。
