• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

提取物的差异生物学效应——对乳腺癌模型的体外研究

Differential Biological Effects of Extracts-In Vitro Studies on Breast Cancer Models.

作者信息

Albulescu Lucian, Suciu Alexandru, Neagu Mihaela, Tanase Cristiana, Pop Sevinci

机构信息

Biochemistry & Proteomics Laboratory, "Victor Babes" National Institute of Pathology, 050096 Bucharest, Romania.

Research Department, SC Hofigal Export-Import SA, 042124 Bucharest, Romania.

出版信息

Antioxidants (Basel). 2024 Nov 22;13(12):1435. doi: 10.3390/antiox13121435.

DOI:10.3390/antiox13121435
PMID:39765764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11672829/
Abstract

The increasing popularity of herbal supplements emphasizes the need of scientific data regarding their health benefits and possible toxicological concerns. The complexity of botanical extracts, which include thousands of distinct compounds, contributes to the challenging nature of this endeavor. In this study, we explored the hormetic effects of two extracts on breast cell lines. Using a wide range of concentrations (0.1 to 3.33 mg/mL), we analyzed how extracts modulate cellular processes such as viability, proliferation, and oxidative stress on breast adenocarcinoma highly invasive estrogen receptor negative (ER-) and noninvasive ER+ cells, as well as on non-tumorigenic ER- normal cells. The cytotoxicity and real-time cell analysis (RTCA) assays showed that both extracts exercised a biphasic dose effect on adenocarcinoma ER+ and normal ER- cell proliferation and oxidative stress. We report a monotonic dose-dependent cytotoxicity on highly invasive adenocarcinoma ER- cells; the induced apoptosis was based on the pro-oxidant activity of extracts. The reactive oxygen species (ROS) generation by high-dose ethanolic extract was observed in all cells, followed by mitochondria dysfunction. Oxidative stress parameters, such as malondialdehyde (MDA) and reduced glutathione (GSH) levels, and superoxide dismutase (SOD) activity were affected. Our study demonstrates that extracts have chemoprevention potential in normal and tumorigenic breast cells by modulating cellular proliferation and oxidative stress.

摘要

草药补充剂越来越受欢迎，这凸显了获取有关其健康益处和可能的毒理学问题的科学数据的必要性。植物提取物的复杂性，其中包括数千种不同的化合物，使得这项工作具有挑战性。在本研究中，我们探讨了两种提取物对乳腺细胞系的 hormetic 效应。我们使用了广泛的浓度范围（0.1至3.33毫克/毫升），分析了提取物如何调节细胞过程，如对高侵袭性雌激素受体阴性（ER-）和非侵袭性ER+乳腺腺癌细胞以及非致瘤性ER-正常细胞的活力、增殖和氧化应激。细胞毒性和实时细胞分析（RTCA）试验表明，两种提取物对腺癌细胞ER+和正常ER-细胞的增殖和氧化应激均表现出双相剂量效应。我们报告了对高侵袭性腺癌ER-细胞的单调剂量依赖性细胞毒性；诱导的细胞凋亡基于提取物的促氧化活性。在所有细胞中均观察到高剂量乙醇提取物产生活性氧（ROS），随后出现线粒体功能障碍。氧化应激参数，如丙二醛（MDA）和还原型谷胱甘肽（GSH）水平以及超氧化物歧化酶（SOD）活性均受到影响。我们的研究表明，提取物可通过调节细胞增殖和氧化应激在正常和致瘤性乳腺细胞中具有化学预防潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/25db7c78058b/antioxidants-13-01435-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/0918522f7d7a/antioxidants-13-01435-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/4a946653dc13/antioxidants-13-01435-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/f808fe5750e6/antioxidants-13-01435-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/645d1967216a/antioxidants-13-01435-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/4a43eafe3315/antioxidants-13-01435-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/19a7a9c7a96d/antioxidants-13-01435-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/b8d8ffe1c10f/antioxidants-13-01435-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/d3ed5e283b47/antioxidants-13-01435-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/adf028af8e3f/antioxidants-13-01435-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/25db7c78058b/antioxidants-13-01435-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/0918522f7d7a/antioxidants-13-01435-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/4a946653dc13/antioxidants-13-01435-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/f808fe5750e6/antioxidants-13-01435-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/645d1967216a/antioxidants-13-01435-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/4a43eafe3315/antioxidants-13-01435-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/19a7a9c7a96d/antioxidants-13-01435-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/b8d8ffe1c10f/antioxidants-13-01435-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/d3ed5e283b47/antioxidants-13-01435-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/adf028af8e3f/antioxidants-13-01435-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7915/11672829/25db7c78058b/antioxidants-13-01435-g010.jpg

相似文献

1
Differential Biological Effects of Extracts-In Vitro Studies on Breast Cancer Models.提取物的差异生物学效应——对乳腺癌模型的体外研究
Antioxidants (Basel). 2024 Nov 22;13(12):1435. doi: 10.3390/antiox13121435.
2
The Effects of L. Sprouts' Phenolic Compounds on Cell Growth and Migration of MDA-MB-231, MCF-7 and HUVEC Cells.龙豆芽酚类化合物对 MDA-MB-231、MCF-7 和 HUVEC 细胞生长和迁移的影响。
Nutrients. 2020 Jan 19;12(1):257. doi: 10.3390/nu12010257.
3
An In Vitro and In Vivo Assessment of Antitumor Activity of Extracts Derived from Three Well-Known Plant Species.三种著名植物提取物抗肿瘤活性的体外和体内评估
Plants (Basel). 2023 Apr 29;12(9):1840. doi: 10.3390/plants12091840.
4
Antiproliferative Effect of Trifolium Pratens L. Extract in Human Breast Cancer Cells.紫花苜蓿提取物对人乳腺癌细胞的增殖抑制作用。
Nutr Cancer. 2019;71(1):128-140. doi: 10.1080/01635581.2018.1521443. Epub 2018 Dec 29.
5
The impact of Trifolium pratense extract on apoptosis and autophagy in NALM-6 cells: implications for B-ALL intervention.红车轴草提取物对 NALM-6 细胞凋亡和自噬的影响：对 B-ALL 干预的意义。
Med Oncol. 2024 Oct 1;41(11):257. doi: 10.1007/s12032-024-02485-4.
6
Cytotoxic and apoptotic effects of Ferula gummosa Boiss: extract on human breast adenocarcinoma cell line.阿魏属 Gumosa 博伊斯提取物对人乳腺癌细胞系的细胞毒性和凋亡作用。
Mol Biol Rep. 2024 Apr 29;51(1):592. doi: 10.1007/s11033-024-09364-1.
7
DESIGNER fraction concept unmasks minor bioactive constituents in red clover (Trifolium pratense L.).设计分数概念揭示了红车轴草（Trifolium pratense L.）中的少量生物活性成分。
Phytochemistry. 2023 Oct;214:113789. doi: 10.1016/j.phytochem.2023.113789. Epub 2023 Jul 21.
8
Comparison of the in vitro estrogenic activities of compounds from hops (Humulus lupulus) and red clover (Trifolium pratense).啤酒花（Humulus lupulus）和红三叶草（Trifolium pratense）中化合物的体外雌激素活性比较。
J Agric Food Chem. 2005 Aug 10;53(16):6246-53. doi: 10.1021/jf050448p.
9
L. (red clover) extract and doxorubicin synergistically inhibits proliferation of 4T1 breast cancer in tumor-bearing BALB/c mice through modulation of apoptosis and increase antioxidant and anti-inflammatory related pathways.红车轴草提取物和阿霉素通过调节细胞凋亡以及增强抗氧化和抗炎相关途径，协同抑制荷瘤BALB/c小鼠中4T1乳腺癌的增殖。
Food Sci Nutr. 2020 Jul 6;8(8):4276-4290. doi: 10.1002/fsn3.1724. eCollection 2020 Aug.
10
Citrus Carotenoid Extracts Promote ROS Accumulation and Induce Oxidative Stress to Exert Anti-Proliferative and Pro-Apoptotic Effects in MDA-MB-231 Cells.柑橘类胡萝卜素提取物促进活性氧积累并诱导氧化应激，从而对MDA-MB-231细胞发挥抗增殖和促凋亡作用。
Antioxidants (Basel). 2024 Feb 22;13(3):264. doi: 10.3390/antiox13030264.

引用本文的文献

1
Phytochemical Profile, Vasodilatory and Biphasic Effects on Intestinal Motility, and Toxicological Evaluation of the Methanol and Dichloromethane Extracts from the Aerial Parts of Used in Traditional Mexican Medicine.墨西哥传统医学中使用的植物地上部分甲醇和二氯甲烷提取物的植物化学特征、对肠道运动的血管舒张和双相作用以及毒理学评价
Pharmaceuticals (Basel). 2025 Jul 30;18(8):1134. doi: 10.3390/ph18081134.

本文引用的文献

1
Current advances and future trends of hormesis in disease.疾病中兴奋效应的当前进展与未来趋势
NPJ Aging. 2024 May 15;10(1):26. doi: 10.1038/s41514-024-00155-3.
2
Phytoestrogens: Chemistry, potential health benefits, and their medicinal importance.植物雌激素：化学、潜在健康益处及其药用重要性。
Phytother Res. 2024 Jun;38(6):3060-3079. doi: 10.1002/ptr.8196. Epub 2024 Apr 11.
3
Reassessing the role of phytochemicals in cancer chemoprevention.重新评估植物化学物质在癌症化学预防中的作用。
Biochem Pharmacol. 2024 Oct;228:116165. doi: 10.1016/j.bcp.2024.116165. Epub 2024 Mar 26.
4
Enemies or Allies? Hormetic and Apparent Non-Dose-Dependent Effects of Natural Bioactive Antioxidants in the Treatment of Inflammation.敌亦或友？天然生物活性抗氧化剂在炎症治疗中的适应原和非剂量依赖性效应。
Int J Mol Sci. 2024 Feb 4;25(3):1892. doi: 10.3390/ijms25031892.
5
Are Supra-Physiological Plant-Based Antioxidants Ready for the Clinic? A Scoping Review of Hormetic Influences Driving Positive Clinical Outcomes.超生理水平的植物性抗氧化剂是否已准备好应用于临床？对驱动积极临床结果的 hormetic 影响的范围审查。
Glob Adv Integr Med Health. 2024 Feb 6;13:27536130241231508. doi: 10.1177/27536130241231508. eCollection 2024 Jan-Dec.
6
Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits.基于植物部位和遗传特性的不同红三叶草基因型的植物化学成分
Foods. 2023 Dec 28;13(1):103. doi: 10.3390/foods13010103.
7
Comparative polyphenolic profiling of five ethnomedicinal plants and their applicative potential in the treatment of type 2 diabetes.五种民族药用植物的多酚特性比较及其在 2 型糖尿病治疗中的应用潜力。
J Ethnopharmacol. 2024 Feb 10;320:117377. doi: 10.1016/j.jep.2023.117377. Epub 2023 Nov 7.
8
Moringa induces its beneficial effect via hormesis.辣木通过诱导适应性反应发挥其有益作用。
Nutr Res Rev. 2024 Dec;37(2):239-248. doi: 10.1017/S0954422423000161. Epub 2023 Sep 4.
9
A Global Overview of Dietary Supplements: Regulation, Market Trends, Usage during the COVID-19 Pandemic, and Health Effects.膳食补充剂的全球概述：监管、市场趋势、在 COVID-19 大流行期间的使用情况以及健康影响。
Nutrients. 2023 Jul 26;15(15):3320. doi: 10.3390/nu15153320.
10
Multidirectional Effects of Red Clover ( L.) in Support of Menopause Therapy.红车轴草（L.）对支持更年期疗法的多向作用。
Molecules. 2023 Jul 3;28(13):5178. doi: 10.3390/molecules28135178.