Suppr超能文献

Protease-deficient Bacillus subtilis host strains for production of Staphylococcal protein A.

作者信息

Fahnestock S R, Fisher K E

出版信息

Appl Environ Microbiol. 1987 Feb;53(2):379-84. doi: 10.1128/aem.53.2.379-384.1987.

Abstract

We constructed strains of Bacillus subtilis which produced very low levels of extracellular proteases. These strains carried insertion or deletion mutations in the subtilisin structural gene (apr) which were constructed in vitro by using the cloned gene. The methods used to construct the mutations involved the use of a plasmid vector which allowed the selection of chromosomal integrates and their subsequent excision by homologous recombination to effect replacement of the chromosomal apr gene by a derivative carrying an inactivating insert with a selectable marker (a cat gene conferring chloramphenicol resistance). The strains produced no subtilisin, no detectable extracellular metalloprotease activity, and residual extracellular serine protease levels as low as 0.5% of that of the standard strain from which they were derived. The strains proved to be superior host strains for the production of staphylococcal protein A, accumulating higher levels of intact protein than do previously available B. subtilis strains.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b1/203669/6f0315dafd59/aem00119-0177-a.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验