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枯草芽孢杆菌细胞外碱性和中性蛋白酶缺陷型双突变体的构建。

Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.

作者信息

Kawamura F, Doi R H

出版信息

J Bacteriol. 1984 Oct;160(1):442-4. doi: 10.1128/jb.160.1.442-444.1984.

DOI:10.1128/jb.160.1.442-444.1984
PMID:6434524
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214740/
Abstract

A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development.

摘要

通过基因转化技术构建了枯草芽孢杆菌的一个突变菌株，该菌株在细胞外中性蛋白酶（nprE）和丝氨酸蛋白酶（aprA）的结构基因中存在损伤。此突变体的细胞外蛋白酶活性不到野生型的4%，且能正常形成芽孢，这表明这些与芽孢形成相关的蛋白酶对芽孢形成均非必需。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1962/214740/d5658e4c7f46/jbacter00227-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1962/214740/d5658e4c7f46/jbacter00227-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1962/214740/d5658e4c7f46/jbacter00227-0453-a.jpg

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