Li Shentang, Shi Ruting, Tian Lang, Chen Jia, Li Xin, Huang Lihua, Yang Zuocheng
a Department of Pediatrics , The Third Xiangya Hospital of Central South University , Changsha , Hunan China.
b Department of Rehabilitation , The Third Xiangya Hospital of Central South University , Changsha , Hunan China.
Immunol Invest. 2019 Feb;48(2):181-189. doi: 10.1080/08820139.2018.1529790. Epub 2018 Oct 15.
Kawasaki disease (KD) is an acute systemic vasculitis that predominantly affects children, and it can result in coronary artery lesions. Cyclooxygenase-2 (COX-2) is involved in the conversion of arachidonic acid to prostaglandin H2, an important precursor of several prostaglandins. The aim of this study was to examine the association between COX-2 gene polymorphisms and susceptibility to KD.
A total of 276 subjects (136 KD and 140 controls) were recruited. The analysis of two single nucleotide polymorphisms rs689466 (-1195G/A) and rs20417 (-765G/C) was respectively detected with polymerase chain reaction sequence-based typing methods.
Polymorphisms of rs689466 were significantly different between the normal controls and KD patients (χ = 6.070 and 5.435, both p < 0.05). The frequencies of AA genotype and A allele of rs689466 in Kawasaki disease group were higher than that of control group (χ = 4.832, p = 0.028, OR = 1.832, 95%CI = 1.064-3.124; χ = 5.435, p = 0.028, OR = 1.491, 95%CI = 1.065-2.088).
This study provides the first evidence supporting an association between COX-2 gene polymorphisms and susceptibility of KD. The AA genotype and A allele of rs689466 confer predisposing factors to KD.
川崎病（KD）是一种主要影响儿童的急性全身性血管炎，可导致冠状动脉病变。环氧化酶-2（COX-2）参与花生四烯酸转化为前列腺素H2，前列腺素H2是几种前列腺素的重要前体。本研究旨在探讨COX-2基因多态性与KD易感性之间的关联。
共招募了276名受试者（136例KD患者和140名对照）。采用基于聚合酶链反应序列的分型方法分别检测两个单核苷酸多态性rs689466（-1195G/A）和rs20417（-765G/C）。
正常对照组与KD患者之间rs689466的多态性存在显著差异（χ² = 6.070和5.435，均p < 0.05）。川崎病组中rs689466的AA基因型和A等位基因频率高于对照组（χ² = 4.832，p = 0.028，OR = 1.832，95%CI = 1.064 - 3.124；χ² = 5.435，p = 0.028，OR = 1.491，95%CI = 1.065 - 2.088）。
本研究提供了首个证据支持COX-2基因多态性与KD易感性之间的关联。rs689466的AA基因型和A等位基因是KD的易感因素。
